New traits to identify physiological responses induced by different rootstocks after root-knot nematode inoculation (Meloidogyne incognita) in sweet pepper
New traits to identify physiological responses induced by different rootstocks after root-knot nematode inoculation (Meloidogyne incognita) in sweet pepper
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DOI：
10.1016/j.cropro.2019.01.026
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发表时间：
2019-05-01
期刊：
影响因子：
2.8
通讯作者：
Lopez-Marin, Josefa
中科院分区：
文献类型：
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作者：
Galvez, Amparo;del Amor, Francisco M.;Lopez-Marin, Josefa
根结线虫（RKNs）是限制全球集约化农业潜在产量的主要植物病理学问题之一。抗性砧木嫁接已被证明是管理几种作物（番茄、甜瓜、西瓜等）的一种可行技术。然而，关于地中海气候条件下温室中甜椒（Capsicum annuum L.）生长的研究很少。因此，我们研究了一个易感接穗品种（Gacela）嫁接到抗性砧木（C19、C25和RT17）上，并与未嫁接和自嫁接（GAL）植株进行了比较。RKN在未嫁接植株和GAL植株中产生显著的根腐蚀；然而，RT17不受影响。从生理学和农艺学的角度讨论了这种对感染的不同反应。因此，接种易感砧木后，气孔调节和光化学效率（叶绿素a荧光）的改变导致叶片光合作用受损，导致果实营养物质浓度（尤其是Ca）不平衡，并增加了光合作用中碳分配给生化防御化合物的生产。因此，总酚类物质和类胡萝卜素受到显著影响，氨基酸也受到显著影响。这些结果为鉴定抗rkn砧材的性状提供了新的见解，因此提供了诱导抗性的新工具，同时避免在土壤中使用消毒剂。
Root-knot nematodes (RKNs) are one of the major phytopathological problems that limit potential yields in intensive agriculture worldwide. Grafting with resistant rootstocks has been demonstrated to be a viable technique to manage Meloidogyne incognita in several crops (tomato, melon, watermelon, etc.). However, little research has been conducted on sweet pepper (Capsicum annuum L.) grown in greenhouses under Mediterranean-climate conditions. Therefore, we studied a susceptible scion variety (Gacela) grafted onto resistant rootstocks (C19, C25, and RT17), in comparison with ungrafted and self-grafted (GAL) plants. The RKN produced significant root galling in ungrafted and GAL plants; however, RT17 was unaffected. This differing response to infection is discussed from physiological and agronomic perspectives. Thus, following the inoculation of susceptible rootstocks, leaf photosynthesis was impaired by altered stomatal regulation and photochemical efficiency (chlorophyll a fluorescence), which led to unbalanced fruit nutrient concentrations (especially Ca) and to enhanced allocation of carbon from photosynthesis to the production of biochemical defense compounds. Consequently, total phenolics and carotenoids were significantly affected, as was the amino acid profile. These results provide new insights into the traits that can identify RKN-resistant rootstocks, and therefore new tools to induce resistance, while avoiding the use of disinfectants in the soil.