Paper-based molecular diagnostic for Chlamydia trachomatis.
Paper-based molecular diagnostic for Chlamydia trachomatis.
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DOI：
10.1039/c4ra07911f
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发表时间：
2014-01-01
期刊：
影响因子：
3.9
通讯作者：
Klapperich CM
中科院分区：
文献类型：
--
作者：
Linnes JC;Fan A;Rodriguez NM;Lemieux B;Kong H;Klapperich CM
在此，我们展示了一种最小仪器化的基于纸的分子诊断的发展，用于沙眼衣原体引起的性传播感染的护理点检测。这种新的诊断平台结合了细胞裂解、等温核酸扩增和侧流视觉检测，仅使用压力源和加热块，无需昂贵的实验室设备。这种基于纸张的测试可以在不到一个小时内进行，并具有临床相关的检测限，比目前用于衣原体诊断的快速免疫测定灵敏100倍。
Herein we show the development of a minimally instrumented paper-based molecular diagnostic for point of care detection of sexually transmitted infections caused by Chlamydia trachomatis. This new diagnostic platform incorporates cell lysis, isothermal nucleic acid amplification, and lateral flow visual detection using only a pressure source and heat block, eliminating the need for expensive laboratory equipment. This paper-based test can be performed in less than one hour and has a clinically relevant limit of detection that is 100x more sensitive than current rapid immunoassays used for chlamydia diagnosis.