FATES OF THE BLASTOMERES OF THE 32-CELL-STAGE XENOPUS EMBRYO
FATES OF THE BLASTOMERES OF THE 32-CELL-STAGE XENOPUS EMBRYO
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DOI：
10.1016/0012-1606(87)90296-x
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发表时间：
1987-08-01
影响因子：
2.7
通讯作者：
MOODY, SA
中科院分区：
文献类型：
--
作者：
MOODY, SA
一个详细的命运地图，所有的后代来自每个卵裂球的32细胞阶段的南非爪蛙胚胎（非洲爪蟾），这是选择刻板分裂，提出。个别卵裂球注射辣根过氧化物酶和所有的后代在后期尾芽胚胎（阶段32至34）进行了鉴定后，组织化学处理的系列组织切片和整装准备。每个卵裂球的后代在表型和位置上都有特征性的分布。大多数器官主要由特定卵裂球集合的后代填充，每个卵裂球的后代通常被限制在定义的空间地址。因此，任何一个卵裂球的后代是不同的和可预测的，当胚胎被预选定型卵裂。然而，胚胎之间的变异是常见的，人们可能期望器官含有来自任何特定卵裂球的后代的频率也有报道。概述了16细胞期卵裂球及其32细胞期子卵裂球的命运差异，并可分为三大类。两个子细胞可以在特定器官中产生相同数量的细胞，一个子细胞可以在来源于母卵裂球的器官中产生更多的细胞，或者一个子细胞可以在来源于母卵裂球的器官中产生所有的后代。因此，细胞命运在卵裂阶段以对称和不对称的方式分离，并且谱系表现出多样化模式（G。S. Stent，1985年，Philos. Trans R. Soc.伦敦系列B 312，3-19）的细胞分裂。
A detailed fate map of all of the progeny derived from each of the blastomeres of the 32-cell-stage South African clawed frog embryo (Xenopus laevis), which were selected for stereotypic cleavages, is presented. Individual blastomeres were injected with horseradish peroxidase and all of their descendants in the late tailbud embryo (stages 32 to 34) were identified after histochemical processing of serial tissue sections and whole-mount preparations. The progeny of each blastomere were distributed characterisically, both in phenotype and location. Most organs were populated largely by the descendants of particular sets of blastomeres, the progeny of each often being restricted to defined spatial addresses. Thus, the descendants of any one blastomere were distinct and predictable when embryos were preselected for stereotypic cleavages. However, variations among embryos were common and the frequencies with which one may expect organs to contain progeny from any particular blastomere are reported. The differences in the fates of the 16-cell-stage blastomeres and their 32-cell-stage daughter blastomeres are outlined and can be grouped into three general categories. The two daughter cells may give rise to equal numbers of cells in a particular organ, one daughter cell may give rise to many more of the cells in an organ derived from the mother blastomere, or one daughter cell may give rise to all of the progeny in an organ derived from the mother blastomere. Thus, cell fates are segregated during cleavage stages in both symmetric and asymmetric manners, and the lineages exhibit a diversification mode (G. S. Stent, 1985, Philos. Trans R. Soc. London Ser. B 312, 3-19) of cell division.