Enteric Populations of Escherichia coli are Likely to be Resistant to Phages Due to O Antigen Production.
Enteric Populations of Escherichia coli are Likely to be Resistant to Phages Due to O Antigen Production.
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由于 O 抗原的产生，大肠杆菌的肠道菌群可能对噬菌体具有抗性。
DOI：
10.1101/2023.11.08.566299
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Woodworth,MichaelH
中科院分区：
文献类型：
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作者：
Berryhill,BrandonA;Burke,KylieB;Fontaine,Jake;Brink,CatherineE;Harvill,MasonG;Goldberg,DavidA;Konstantinidis,KonstantinosT;Levin,BruceR;Woodworth,MichaelH
大量的生物信息学数据表明，噬菌体在人类肠道微生物群中大量存在。这些病毒在塑造肠道微生物群的细菌菌株和物种组成方面的贡献是什么？这些噬菌体是如何随着时间的推移而维持的？为了解决这些问题，我们将大肠杆菌和噬菌体从四种粪便微生物群移植（FMT）剂量中分离出来作为非生态失调肠道微生物群的代表性样本进行了实验，并建立和分析了细菌和噬菌体种群和进化动力学的数学模型的特性。我们的模型预测和实验证实，由于O抗原的产生，肠道微生物群中的大肠杆菌可能对共存的噬菌体感染具有抵抗力。此外，我们的模型表明，由于宿主在抗性和敏感状态之间转换的高速率，噬菌体可以在群体中维持，我们称之为泄漏抗性。根据我们的观察和模型预测，我们假设在人类肠道中发现的噬菌体在塑造健康个体肠道微生物组中大肠杆菌菌株水平的组成方面可能起不到什么作用。尽管O抗原表达在许多分类群中很常见，但对于肠道菌群中其他种类的细菌来说，这种情况有多普遍尚不清楚。
There is a surfeit of bioinformatic data showing that bacteriophages abound in the enteric microbiomes of humans. What is the contribution of these viruses in shaping the bacterial strain and species composition of the gut microbiome and how are these phages maintained over time? To address these questions, we performed experiments with Escherichia coli and phages isolated from four fecal microbiota transplantation (FMT) doses as representative samples of non-dysbiotic enteric microbiota and develop and analyze the properties of a mathematical model of the population and evolutionary dynamics of bacteria and phage. Our models predict and experiments confirm that due to production of the O antigen, E. coli in the enteric microbiome are likely to be resistant to infection with co-occurring phages. Furthermore, our modeling suggests that the phages can be maintained in the population due to the high rates of host transition between resistant and sensitive states, which we call leaky resistance. Based on our observations and model predictions, we postulate that the phages found in the human gut are likely to play little role in shaping the composition of E. coli at the strain level in the enteric microbiome in healthy individuals. How general this is for other species of bacteria in the enteric flora is not yet clear, although O antigen expression is common across many taxa.