Cytotactin and its proteoglycan ligand mark structural and functional boundaries in somatosensory cortex of the early postnatal mouse.
Cytotactin and its proteoglycan ligand mark structural and functional boundaries in somatosensory cortex of the early postnatal mouse.
复制标题
细胞趋化素及其蛋白聚糖配体标记了出生后早期小鼠体感皮层的结构和功能边界。
DOI：
10.1016/0012-1606(89)90264-9
复制
发表时间：
1989
影响因子：
2.7
通讯作者：
Edelman,GM
中科院分区：
文献类型：
--
作者：
Crossin,KL;Hoffman,S;Tan,SS;Edelman,GM
细胞外基质分子cytokrin，这是由神经胶质细胞合成的，和细胞粘附素结合（CTB）蛋白聚糖，这是由神经元合成的表达，在小鼠的大脑发育中，特别是在皮质桶场，使用高度特异性的多克隆抗体纯化的分子。这两种分子出现在早期的发展中的皮层，但被排除在外的中心的发展桶在进入和树枝状的丘脑皮层轴突的时间。的两种主要形式的细胞色素（220和200 kDa），较大的形式占主导地位，在小鼠大脑的发展过程中，也占主导地位的混合神经元-神经胶质细胞文化，但不是在纯神经胶质细胞文化。细胞凝集素和CTB蛋白聚糖都被各种凝集素所识别，这些凝集素在其他研究中已被证明可以划分桶场：这两种分子都被小扁豆凝集素和伴刀豆球蛋白A所识别，CTB蛋白聚糖也被花生和小麦胚芽凝集素所识别。HNK-1碳水化合物抗原，存在于胞浆素，CTB蛋白聚糖，和其他粘附分子，也被发现在桶壁和减少桶中空。胞浆素和CTB蛋白聚糖优先表达在桶壁通过P12。在此之后，它们的表达变得均匀，即使保持桶壁和空洞的组织学模式。一排桶，这是从外围损坏的一排胡须的融合伴随着两种分子的模式表达的损失后，在P1.5的一排胡须毛囊电灼。我们的结论是，从周边的活动是重要的，不仅发展的解剖模式，但也的分子模式和神经胶质细胞和神经元蛋白的表达可以响应这种活动。结果是一致的，与以前的研究表明，传入丘脑皮层轴突在桶场形成中发挥了主要作用。他们还建议，无论是迁移的皮层神经元上的胶质细胞和细化的周边晶须领域和其皮质代表之间的映射可能取决于基板粘附分子的分布。
The expression of the extracellular matrix molecules cytotactin, which is synthesized by glia, and cytotactin-binding (CTB) proteoglycan, which is synthesized by neurons, was examined in the developing brain of the mouse, specifically in the cortical barrel field, using highly specific polyclonal antibodies to the purified molecules. Both molecules appeared early in the development of the cortex but were excluded from the centers of the developing barrels at the time of entry and arborization of thalamocortical axons. Of the two major forms of cytotactin (220 and 200 kDa), the larger form predominated during development of the mouse brain and also predominated in mixed neuron-glia cultures but not in pure glial cultures. Both cytotactin and CTB proteoglycan were recognized by various lectins that have been shown in other studies to demarcate the barrel field: both molecules were recognized by lentil lectin and concanavalin A and CTB proteoglycan was also recognized by peanut and wheat germ agglutinins. The HNK-1 carbohydrate antigen, present on cytotactin, CTB proteoglycan, and other adhesion molecules, was also found in the barrel walls and diminished in the barrel hollows. Cytotactin and CTB proteoglycan were preferentially expressed in barrel walls through P12. After this time, their expression became uniform even though the histological pattern of barrel walls and hollows was maintained. The fusion of a row of barrels which results from peripheral damage to a row of whiskers was accompanied by the loss of patterned expression of both molecules following electrocauterization of a row of whisker follicles at P1.5. We conclude that activity from the periphery is important not only to development of anatomical pattern but also of the molecular pattern and that the expression of both glial and neuronal proteins can respond to such activity. The results are consistent with previous studies showing that incoming thalamocortical axons play a primary role in barrel field formation. They also suggest that both the migration of cortical neurons on glia and the refinement of the mapping between the peripheral whisker field and its cortical representation may depend upon the distribution of substrate adhesion molecules.