Evidence for a functional link between Dd-STATa and Dd-PIAS, a Dictyostelium PIAS homologue
Evidence for a functional link between Dd-STATa and Dd-PIAS, a Dictyostelium PIAS homologue
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Dd-STATa 和 Dd-PIAS（盘基网柄菌 PIAS 同源物）之间功能联系的证据
DOI：
10.1111/j.1440-169x.2011.01296.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
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作者：
Kawata;T.;Hirano;T.;Ogasawara;S.;Aoshima;R. and Yachi;A.
一些哺乳动物蛋白家族抑制信号转导蛋白和转录激活蛋白 (STAT) 的活性。活化 STAT 蛋白抑制剂 (PIAS) 最初是通过其与人类 STAT 蛋白相互作用的能力而被识别的。我们分离出一个编码PIAS的网网柄菌直系同源基因（pisA），Dd-PIAS，它几乎拥有哺乳动物PIAS蛋白的所有代表性基序和结构域。 ADd-PIASnull 突变株表现出正常的末端形态，但一旦细胞聚集，就会加速发育。相比之下，Dd-PIAS 过表达菌株表现出聚集延迟、几乎没有蛞蝓趋光性、蛞蝓运动性受损以及蛞蝓迁移期延长。该菌株是 Dd-STATanull 突变体的近表型，尽管它最终形成子实体，尽管效率低下。在 Dd-PIAS 无效突变体中，几个 Dd-STATa 激活基因的表达上调，并且 pstAB 标记存在异位表达。在 PIAS 启动子下表达的 PIAS-绿色荧光蛋白 (GFP) 融合蛋白的浓度在 pstO 细胞中最高，并随着接近蛞蝓和顶点的尖端逐渐降低：与 Dd-STATa 的模式完全相反。我们的结果表明 Dd-PIAS 和 Dd-STATa 之间存在影响基因表达和发育的功能相互关系。
Several mammalian protein families inhibit the activity of signal transducer and activator of transcription (STAT) proteins. The protein inhibitor of activated STAT (PIAS) was initially identified through its ability to interact with human STAT proteins. We isolated a gene (pisA) encoding aDictyosteliumorthologue of PIAS, Dd‐PIAS, which possesses almost all the representative motifs and domains of mammalian PIAS proteins. ADd‐PIASnull mutant strain displays a normal terminal morphology but with accelerated development once cells are aggregated. In contrast, Dd‐PIAS overexpressor strains demonstrate delayed aggregation, almost no slug phototaxis, impaired slug motility, and a prolonged slug migration period. This strain is a near phenocopy of theDd‐STATanull mutant, although it eventually forms a fruiting body, albeit inefficiently. The expression of several Dd‐STATa‐activated genes is upregulated in theDd‐PIASnull mutant and there is ectopic expression of pstAB makers. The concentration of a PIAS‐green fluorescent protein (GFP) fusion protein, expressed under the PIAS promoter, is greatest in the pstO cells and gradually decreases with proximity to the tip of the slug and culminant: a pattern diametrically opposite to that of Dd‐STATa. Our results suggest a functional interrelationship between Dd‐PIAS and Dd‐STATa that influences gene expression and development.