Expression of Aromatase mRNA and effects of aromatase inhibitor during ovarian development in the medaka, Oryzias latipes
Expression of Aromatase mRNA and effects of aromatase inhibitor during ovarian development in the medaka, Oryzias latipes
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DOI：
10.1002/jez.a.20027
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发表时间：
2004-03-01
影响因子：
2.8
通讯作者：
Shibata, N
中科院分区：
文献类型：
--
作者：
Suzuki, A;Tanaka, M;Shibata, N
在硬骨鱼中，一些研究表明雌激素在卵巢发育过程中起作用，但内源性雌激素的确切作用仍不清楚。我们用原位杂交技术检测了青鳉卵巢发育过程中芳香化酶mRNA的表达，以及非甾体芳香化酶抑制剂法倔唑（Fadrozole）的作用。在卵子发生后4至10天（dah），首次在卵巢腹侧检测到青鳉芳香酶。孵化后AI治疗抑制卵巢腔形成从30 dah，但不影响卵巢发育过程中的早期卵子发生和卵泡发生。这些结果表明，内源性雌激素是特别需要的卵巢腔的形成，但不是必不可少的早期卵子发生和卵泡发育青鳉。(C)2004 Wiley-Liss，Inc.
In teleost fish, several studies have implicated estrogens in the process of ovarian development, but the exact role of endogenous estrogen is still unclear, We examined the expression of aromatase mRNA with in situ hybridization, and the effects of Fadrozole, a nonsteroidal aromatase inhibitor (AI), during ovarian development in medaka Oryzias latipes. Medaka aromatase was first detected on the ventral side of ovaries from four to 10 days after hatching (dah), after occurrence of oogenesis. AI treatment after hatching suppressed the ovarian cavity formation from 30 dah but did not affect early oogenesis and folliculogenesis during ovarian development. These results suggest that endogenous estrogen is specifically required for formation of the ovarian cavity, but is not essential for early oogenesis and folliculogenesis in medaka. (C) 2004 Wiley-Liss, Inc.