Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy
Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy
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DOI：
10.1016/j.neuroimage.2010.03.065
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发表时间：
2010-08-15
期刊：
影响因子：
5.7
通讯作者：
Chen, You-Yin
中科院分区：
文献类型：
--
作者：
Liao, Lun-De;Li, Meng-Lin;Chen, You-Yin
本研究报道了一种新型的功能性光声显微镜（fPAM）系统的发展，该系统用于研究与电前爪刺激相关的大鼠皮质血管的血流动力学变化。利用fPAM在多个适当选择的不同波长下对血液光吸收成像，可以探测总血红蛋白浓度（HbT，即脑血容量[CBV]）和血红蛋白氧饱和度（SO2）的变化。CBV的变化是通过波长为570nm （lambda(570)）的图像测量的，这是氧和脱氧血红蛋白摩尔消光光谱的等吸点，而SO2的变化是通过将波长为lambda（560）或lambda（600）的图像与波长为lambda（570）的图像逐像素归一化来感知的。我们证明了fPAM系统能够成像和量化在前爪电刺激下对侧SO2和CBV的显著变化。fPAM系统补充了现有的成像技术，有可能成为动物模型中明确研究脑血流动力学的有利工具。(C) 2010爱思唯尔公司版权所有。
The present study reported the development of a novel functional photoacoustic microscopy (fPAM) system for investigating hemodynamic changes in rat cortical vessels associated with electrical forepaw stimulation. Imaging of blood optical absorption by fPAM at multiple appropriately-selected and distinct wavelengths can be used to probe changes in total hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) and hemoglobin oxygen saturation (SO2). Changes in CBV were measured by images acquired at a wavelength of 570 nm (lambda(570)), an isosbestic point of the molar extinction spectra of oxy- and deoxy-hemoglobin, whereas SO2 changes were sensed by pixel-wise normalization of images acquired at lambda(560) or lambda(600) to those at lambda(570). We demonstrated the capacity of the fPAM system to image and quantify significant contralateral changes in both SO2 and CBV driven by electrical forepaw stimulation. The fPAM system complements existing imaging techniques, with the potential to serve as a favorable tool for explicitly studying brain hemodynamics in animal models. (C) 2010 Elsevier Inc. All rights reserved.