Trehalose drastically extends the in vitro vegetative culture period and facilitates maintenance of Torenia fournieri plants
Trehalose drastically extends the in vitro vegetative culture period and facilitates maintenance of Torenia fournieri plants
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DOI：
10.5511/plantbiotechnology.11.0124c
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Ohtsubo, Norihiro
中科院分区：
文献类型：
--
作者：
Yamaguchi, Hiroyasu;Sasaki, Katsutomo;Ohtsubo, Norihiro
而Torenia(Torenia ournieri Lind.)是花卉构筑的分子生物学研究的有用模式花，由于其杂合的性质，植物材料的维持和产生的转基因植物需要营养繁殖。因此，减少劳动力和维持数以千计的托雷尼亚体外培养的成本是一个关键问题。我们发现，用海藻糖替代蔗糖可以显著延长培养周期至70天，这是普通蔗糖为基础的培养液的两倍多，而不会降低植物的生存能力。对植物质量的比较测量表明，以海藻糖为基础的基质的生存效益的增加可能是因为通过减少培养中的根密度来改善根际环境，而不是通过减少植物的生长。在超过12个月的双月继代培养过程中，1800个实验室品系中没有观察到海藻糖基基的有害影响，除了第一次转移到海藻糖基基上时会枯萎。结合使用商业食品添加剂Okome-Ni-TREHA(R)而不是试剂级海藻糖，我们成功地将与培养介质相关的成本和劳动力减少到不到蔗糖系统的三分之一。
While torenia (Torenia fournieri Lind.) is a useful model flower for molecular biological studies of floral architecture, the maintenance of plant materials and resulting transgenic plants requires vegetative propagation due to its heterozygous nature. Reduction of labor and costs for maintaining thousands of in vitro torenia cultures is therefore a critical issue. We found that substituting trehalose for sucrose drastically extended the culture period to 70 days, which is more than twice as long as for the common, sucrose-based medium, without reduction in plant viability. Comparative measurement of the plant mass indicated that the increased survival benefit of the trehalose-based medium might be on account of improvement in the rhizosphere environment through reduction of root density in the culture, rather than by reduced plant growth. No harmful effects arising from the trehalose-based medium were observed in 1,800 laboratory lines during the bimonthly subculture for over 12 months, except for a wilting on the first transfer to the trehalose-based medium. In conjunction with the use of the commercial food additive, Okome-ni-TREHA (R) rather than reagent-grade trehalose, we have succeeded in reducing the costs and labor associated with the culture medium to less than one third of those for the sucrose-based system.