Towards a New Reference Test for Surra in Camels
Towards a New Reference Test for Surra in Camels
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DOI：
10.1128/cvi.00096-09
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发表时间：
2009-07-01
影响因子：
--
通讯作者：
Buescher, Philippe
中科院分区：
文献类型：
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作者：
Tran, Thao;Claes, Filip;Buescher, Philippe
目前骆驼伊氏锥虫感染的血清学诊断基于天然可变抗原类型 RoTat 1.2。这项研究的目标是开发一种基于不变蛋白质的新型血清学诊断测试，并且无需使用大鼠或小鼠进行生产。使用不变表面糖蛋白 75 的重组胞外域 (ELISA/rISG75) 开发了酶联免疫吸附测定法，并在 184 份骆驼血清集合上进行了测试。将结果与基于可变表面糖蛋白 RoTat 1.2 的三种已建立的抗体检测测试获得的结果进行比较：伊氏锥虫 ELISA (ELISA/T. evansi)、锥虫病卡凝集测试 (CATT/T. evansi) 和免疫锥虫溶解 (TL) 测定。 ELISA/rISG75 和 ELISA/T。 evansi 显示的敏感性分别为 94.6%（95% 置信区间 [CI]，87.8 至 98.2%，19% 阳性截止值）和 98.9%（95% CI，94.1 至 99.8，12% 阳性截止值）。 ELISA/rISG75 具有 100% 特异性（CI，95.9 至 100%），而 ELISA/T. evansi 显示出 98.9% 的特异性（CI，95.9 至 100%）。 ELISA/rISG75 证明与 TL 测定（CATT/T）几乎完美一致。埃文西和 ELISA/T。 evansi，kappa 分数至少为 0.94。 ELISA/rISG75 具有与金标准（TL 测定）相当的性能，并且不受抗原变异的影响，可能成为骆驼 surra 的新参考测试。它为诊断其他宿主的伊氏锥虫感染以及开发用于检测布氏锥虫、T. b. 的泛锥虫测试开辟了途径。冈比亚，T. b. rhodesense、T. evansi 和 T.Equiperdum。
Current serological diagnosis of Trypanosoma evansi infection in camels is based on the native variable antigen type RoTat 1.2. The goal of this study was to develop a novel serological diagnostic test based on a nonvariable protein and freed from the use of rats or mice for its production. An enzyme-linked immunosorbent assay using a recombinant extracellular domain of invariant surface glycoprotein 75 (ELISA/rISG75) was developed and tested on a collection of 184 camel sera. The results were compared to those obtained from three established antibody detection tests based on variable surface glycoprotein RoTat 1.2: an ELISA for T. evansi (ELISA/T. evansi), a card agglutination test for trypanosomiasis (CATT/T. evansi), and an immune trypanolysis (TL) assay. The ELISA/rISG75 and the ELISA/T. evansi showed a sensitivity of 94.6% (95% confidence interval [CI], 87.8 to 98.2%, at 19% positivity cutoff value) and 98.9% (95% CI, 94.1 to 99.8, at 12% positivity cutoff value), respectively. The ELISA/rISG75 had 100% specificity (CI, 95.9 to 100%), while the ELISA/T. evansi showed 98.9% specificity (CI, 95.9 to 100%). The ELISA/rISG75 demonstrated an almost perfect agreement with the TL assay, the CATT/T. evansi, and the ELISA/T. evansi, with kappa scores of at least 0.94. The ELISA/rISG75, having a performance comparable to that of the gold standard (the TL assay) and being independent of antigenic variation, may become a new reference test for surra in camels. It opens avenues for the diagnosis of T. evansi infections in other hosts as well as for the development of a pan-Trypanozoon test for detection of Trypanosoma brucei brucei, T. b. gambiense, T. b. rhodesiense, T. evansi, and T. equiperdum.