ULTRAMICROCHEMICAL DETERMINATION OF NUCLEIC-ACIDS IN INDIVIDUAL CELLS USING ZEISS UMSP-1 MICROSPECTROPHOTOMETER - APPLICATION TO ISOLATED RAT HEPATOCYTES OF DIFFERENT PLOIDY CLASSES
ULTRAMICROCHEMICAL DETERMINATION OF NUCLEIC-ACIDS IN INDIVIDUAL CELLS USING ZEISS UMSP-1 MICROSPECTROPHOTOMETER - APPLICATION TO ISOLATED RAT HEPATOCYTES OF DIFFERENT PLOIDY CLASSES
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DOI：
10.1007/bf01003963
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
ROOZEMOND, RC
中科院分区：
文献类型：
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作者：
ROOZEMOND, RC
Edstroem''s method for the ultramicrochemical determination of RNA and DNA in individual cells was modified for the measurement of extinction in UV light with the aid of the Zeiss scanning microspectrophotometer UMSP-I. With this new procedure, nucleic acids down to about 3 pg RNA or about 4 pg DNA can be measured with a high accuracy. The method was applied to enzymatically isolated rat liver parenchymal cells. A mean DNA content of 6.52 pg was found for diploid cells. The DNA content of mononuclear cells of different ploidy levels and of binuclear cells showed a close proportionality with the nuclear ploidy and the number of nuclei per cell. The RNA content of mononuclear diploid cells amounted to 33.4 pg, yielding an RNA/DNA ratio of 5.12. The RNA/DNA ratio was similar for binuclear and mononuclear cells of the same ploidy level but decreased considerably with increasing nuclear ploidy.
