Two second messengers mediate amino acid responses in olfactory sensory neurons of the salamander, Necturus maculosus.
Two second messengers mediate amino acid responses in olfactory sensory neurons of the salamander, Necturus maculosus.
复制标题
两个第二信使介导蝾螈 Necturus maculosus 嗅觉感觉神经元的氨基酸反应。
DOI：
10.1093/chemse/27.8.673
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发表时间：
2002
期刊：
影响因子：
3.5
通讯作者：
Dionne,VincentE
中科院分区：
文献类型：
--
作者：
Delay,RonaJ;Dionne,VincentE
腺苷酸环化酶/cAMP通路介导的气味转导已得到很好的研究，但仍不确定该通路是否介导脊椎动物所有气味的转导。我们从斑节蝾螈中分离出嗅觉感觉神经元，并使用钙离子成像和指示染料Fura-2来检测氨基酸引起的嗅觉反应。大约三分之二的气味反应的性质表明，它们是由腺苷酸环化酶/cAMP途径介导的，但三分之一的反应既没有被cAMP类似物模仿，也没有被腺苷酸环化酶的抑制所阻断，这表明这些气味反应的介导方式不同。不受腺苷酸环化酶抑制影响的反应被磷脂酶C抑制剂新霉素阻断，这意味着它们是通过磷脂酶C的激活转导的。一些对一种以上氨基酸产生反应的细胞似乎同时使用了两种途径，但每种途径都被用来吸收不同的气味。此外，许多反应，腺苷酸环化酶/cAMP途径介导的磷脂酶C的抑制后增强，这表明磷脂酶C途径不仅在气味转导的作用，但也在调制的嗅觉反应。
Odor transduction mediated by the adenylyl cyclase/cAMP pathway has been well studied, but it is still uncertain whether this pathway mediates the transduction of all odors in vertebrates. We isolated olfactory sensory neurons from the salamanderNecturus maculosusand used calcium imaging with the indicator dye fura-2 to examine olfactory responses elicited by amino acids. The properties of approximately two-thirds of the odor responses suggested they were mediated by the adenylyl cyclase/cAMP pathway, but one-third of the responses were not mimicked by cAMP analogs nor blocked by inhibition of adenylyl cyclase, suggesting that these odor responses were mediated differently. Responses that were unaffected by inhibition of adenylyl cyclase were blocked by neomycin, an inhibitor of phospholipase C, implying that they were transduced by activation of phospholipase C. Some cells which responded to more than one amino acid appeared to employ both pathways, but each was used to transduce different odors. In addition, many responses that were mediated by the adenylyl cyclase/cAMP pathway were enhanced following inhibition of phospholipase C, suggesting that the phospholipase C pathway has a role not only in odor transduction, but also in the modulation of olfactory responses.