Association between RCAS1 expression and microenvironmental immune cell death in uterine cervical cancer
Association between RCAS1 expression and microenvironmental immune cell death in uterine cervical cancer
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DOI：
10.1016/j.ygyno.2005.02.025
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发表时间：
2005-06-01
影响因子：
4.7
通讯作者：
Nakano, H
中科院分区：
文献类型：
--
作者：
Sonoda, K;Miyamoto, S;Nakano, H
Objective.区域淋巴结转移是影响宫颈癌预后的因素之一。转移的发展要求癌细胞避免淋巴细胞攻击。淋巴细胞功能受损由凋亡因子介导，包括SiSo细胞上表达的受体结合癌抗原（RCAS I）、Fas配体（FasL）和肿瘤坏死因子-α（TNF-α）。我们的目的是评估这些因子的表达和微环境淋巴细胞凋亡在这种疾病之间的关联。采用免疫组化方法检测RCAS 1、FasL和TNF-α在宫颈癌原发灶和转移淋巴结中的表达，并分析其与淋巴细胞凋亡的关系。原发灶和转移淋巴结中RCASI的表达有显著性差异（P < 0.0001），而FasL和TNF-α的表达无显著性差异（P> 0.05）。RCASI阳性表达细胞数与宫颈癌及转移淋巴结中凋亡淋巴细胞数显著相关（P < 0.0001），而FasL、TNF-α阳性表达细胞数与宫颈癌及转移淋巴结中凋亡淋巴细胞数无相关性（P> 0.05）。RCASI的表达可能与宫颈癌原发灶和转移淋巴结中肿瘤细胞通过诱导淋巴细胞凋亡逃避免疫监视有关。(c)2005年爱思唯尔公司All rights reserved.
Objective. The presence of regional lymph node metastasis is one of the prognostic factors for uterine cervical cancer. The development of metastasis requires that cancer cells avoid lymphocyte attack. Impaired lymphocyte function is mediated by apoptotic factors including receptor-binding cancer antigen expressed on SiSo cells (RCAS I), Fas ligand (FasL), and tumor necrosis factor-alpha (TNF-alpha). Our aim was to evaluate the association between expression of these factors and microenvironmental lymphocyte apoptosis in this disease.Methods. Imummohistochemical methods were used to determine the relationship between the expression of RCAS1, FasL, and TNF-alpha, and the number of apoptotic lymphocytes in primary lesions and metastatic lymph nodes in patients with cervical cancer.Results. Expression of these apoptosis-inducing molecules was quite different in primary tumors and metastatic lymph nodes: RCASI expression in lymph nodes was significantly higher than that in primary lesions (P < 0.0001), whereas FasL and TNF-alpha expressions at these two locations were not significantly different. The number of cells with positive expression of RCASI, but not of FasL or TNF-alpha, was significantly correlated with the number of apoptotic lymphocytes in uterine cervix and metastatic lymph nodes (P < 0.0001 for both).Conclusion. RCASI expression may be related to tumor cell evasion of immune surveillance via induction of lymphocyte apoptosis in primary lesions and metastatic lymph nodes in uterine cervical cancer. (c) 2005 Elsevier Inc. All rights reserved.