Development of an immunochromatographic test with recombinant BgSA1 for the diagnosis of Babesia gibsoni infection in dogs
Development of an immunochromatographic test with recombinant BgSA1 for the diagnosis of Babesia gibsoni infection in dogs
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DOI：
10.1007/s00436-006-0392-8
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发表时间：
2007-05-01
影响因子：
2
通讯作者：
Xuan, Xuenan
中科院分区：
文献类型：
--
作者：
Jia, Honglin;Liao, Min;Xuan, Xuenan
本文建立了一种利用重组BgSA1（rBgSA1）检测吉氏巴氏巴氏杆菌抗体的免疫层析试验（ICT），并对其进行了评价。仅采集感染B的犬的血清样品。吉氏血吸虫病阳性，但感染密切相关寄生虫的犬和健康犬的血清标本均为阴性。在实验感染B的狗中可检测到特异性抗体。在急性和慢性感染阶段的ICT。为评价ICT的临床应用，采用ICT和先前建立的rBgSA 1酶联免疫吸附试验（ELISA）检测了从日本家犬中采集的共计94份血清样本。21个测试样品（22个）ICT和ELISA均为阳性者占3%。ELISA法与ICT法的符合率为95.8%。结果表明，rBgSA 1 ICT快速、简便、准确，适用于B的诊断。在野外犬只的吉氏感染。
An immunochromatographic test (ICT) using recombinant BgSA1 (rBgSA1) for the detection of antibodies against Babesia gibsoni was developed and evaluated. Only the serum samples collected from dogs infected with B. gibsoni were positive in the ICT, but the serum samples from dogs infected with closely related parasites and from healthy dogs were negative. The specific antibodies could be detected in a dog experimentally infected with B. gibsoni at both the acute and chronic infection stages by the ICT. To evaluate the clinical application of the ICT, a total of 94 serum samples collected from domestic dogs in Japan were tested with the ICT and the previously established enzyme-linked immunosorbent assay (ELISA) with rBgSA1. Twenty-one of the tested samples (22..3%) were positive in both the ICT and the ELISA. The concordance between the ELISA and the ICT was found to be 95.8%. These results suggested that the ICT using rBgSA1 is rapid, simple, accurate, and suitable for the diagnosis of B. gibsoni infection of dogs in the field.