Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition
Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition
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平味散通过调节肠道菌群组成缓解小鼠慢性结肠炎
DOI：
10.1016/j.jep.2020.112715
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发表时间：
2020
影响因子：
5.4
通讯作者：
Naisheng Zhang
中科院分区：
文献类型：
--
作者：
Zecai Zhang;Hongyang Cao;Peng Shen;Jiuxi Liu;Yongguo Cao;Naisheng Zhang
平胃散（PWS）是一种复杂的中药制剂，最早记载于公元1107年，发表于《太平仁政方书》。我们之前已经证实PWS具有减轻dss诱导的小鼠慢性溃疡性结肠炎（UC）的作用。研究目的我们旨在研究PWS是否通过调节肠道菌群组成来保护小鼠慢性UC。材料与方法C57BL/6小鼠慢性结肠炎实验采用2.5% DSS饮水诱导。实验全程口服PWS （8 g/kg）。测量了这些小鼠的体重变化、粪便稠度和髓过氧化物酶（MPO）活性。采用qRT-PCR检测白细胞介素17a （IL-17A）和干扰素γ (IFN-γ) mRNA水平。采用16S rRNA测序技术研究粪便菌群的变化。检测小肠紧密连接蛋白（occludin）和血清脂多糖（LPS）水平。结果spws减轻了dss引起的小鼠体重下降，改善了粪便一致性和MPO活性。经PWS治疗后，IL-17A和IFN-γ mRNA水平也降低。PWS不仅能调节occludin水平，还能降低血清LPS水平。我们进一步发现dss引起的肠道微生物组成和丰富度的变化受到PWS的显著调节。在DSS诱导的慢性UC小鼠中，PWS治疗显著降低拟杆菌门菌的丰度，但增加了细菌门菌的丰度。结论结合前期研究结果，PWS可通过平衡肠道菌群发挥抗uc作用。
Ethnopharmacological relevancePing weisan(PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary. We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.Aim of the studyWe aimed to examine whether PWS protects mice from chronic UC by regulating intestinal microbiota composition.Materials and methodsChronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water. PWS (8 g/kg) was orally administered throughout the experiment. Body weight changes, stool consistency and myeloperoxidase (MPO) activity were measured in these mice. Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels were detected by qRT-PCR. The alterations of fecal microflora were investigated by 16S rRNA sequencing. Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.ResultsPWS relieved DSS-induced loss of body weight, and improved stool consistency and MPO activity in mice. The levels of IL-17A and IFN-γ mRNA were also reduced after treatment with PWS. PWS not only regulated occludin level but also decreased serum LPS. We further showed DSS-induced changes in intestinal microbial composition and richness are significantly regulated by PWS. PWS treatment significantly decreased the abundance ofBacteroidetes, but increased the abundance ofFirmicutesin chronic UC mice induced by DSS.ConclusionsCombining with our previous results, we found that PWS could exert anti-UC role by rebalancing intestinal bacteria.