High-level expression of alternative oxidase protein sequences enhances the spread of viral vectors in resistant and susceptible plants
High-level expression of alternative oxidase protein sequences enhances the spread of viral vectors in resistant and susceptible plants
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DOI：
10.1099/vir.0.80385-0
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发表时间：
2004-12-01
影响因子：
3.8
通讯作者：
Carr, JP
中科院分区：
文献类型：
--
作者：
Murphy, AM;Gilliland, A;Carr, JP
替代氧化酶（AOX）是植物抗氰化物替代呼吸途径的末端氧化酶，与病毒抗性有关。当烟草花叶病毒（TMV）载体驱动AOX或其活性位点突变的AOX （AOX- e）的高水平表达时，病毒的传播增强。结果显示，在nn基因型烟草上接种后，产生的超敏反应病变比含有相当长度序列的载体（绿色荧光蛋白（gfp）基因序列或反义aox）或“空”病毒载体产生的病变更大。此外，在高易感宿主benthamiana中，表达AOX或AOX- e的TMV载体的全身运动速度比含有gfp或反义AOX序列的TMV构建体快。值得注意的是，在N. benthamiana， TMV。AOX和TMV。AOX-E诱导的症状很严重，最终包括细胞死亡，而空载体TMV。GFP和表达反义aox序列的TMV载体均未诱导坏死。结果表明，AOX活性蛋白和失活蛋白在足够高的表达水平下，可以影响病毒在植物中的传播和症状。
The alternative oxidase (AOX) is the terminal oxidase of the cyanide-resistant alternative respiratory pathway in plants and has been implicated in resistance to viruses. When tobacco mosaic virus (TMV) vectors were used to drive very high levels of expression of either AOX or AOX mutated in its active site (AOX-E), virus spread was enhanced. This was visualized as the induction of larger hypersensitive-response lesions after inoculation onto NN-genotype tobacco than those produced by vectors bearing sequences of comparable length [the green fluorescent protein (gfp) gene sequence or antisense aox] or the 'empty' viral vector. Also, in the highly susceptible host Nicotiana benthamiana, systemic movement of TMV vectors expressing AOX or AOX-E was faster than that of TMV constructs bearing gfp or antisense aox sequences. Notably, in N. benthamiana, TMV.AOX and TMV.AOX-E induced symptoms that were severe and ultimately included cell death, whereas the empty vector, TMV.GFP and the TMV vector expressing antisense aox sequences never induced necrosis. The results show that, if expressed at sufficiently high levels, active and inactive AOX proteins can affect virus spread and symptomology in plants.