The bacterial microbiome of field-collected Dermacentor marginatus and Dermacentor reticulatus from Slovakia
The bacterial microbiome of field-collected Dermacentor marginatus and Dermacentor reticulatus from Slovakia
复制标题
斯洛伐克现场采集的边缘革螨和网纹革螨的细菌微生物组
DOI：
10.1186/s13071-019-3582-9
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发表时间：
2019-06-27
影响因子：
3.2
通讯作者：
Liu, Jing-Ze
中科院分区：
文献类型：
--
作者：
Zhang, Yan-Kai;Yu, Zhi-Jun;Liu, Jing-Ze
背景：微生物区系在硬蜱生物学和生态学中的重要作用已引起人们的广泛关注。在欧洲，包括斯洛伐克在内，已知的边缘德马康特和网状德马康特是各种病原体的传播媒介。方法：利用16S rRNA高通量测序技术对斯洛伐克野外采集的边缘毛滴虫和网纹夜蛾的微生物群落进行了分析。结果：不同的分析结果表明，边缘毛滴虫和网纹夜蛾的微生物群落在多样性和分类结构上存在差异。此外，在这两个物种中检测到了特定物种和性别的细菌。一种可能的细菌病原体“念珠菌Rhabdochlamydia sp.”从边缘毛囊线虫雄性体内检出。在观察到的细菌中，立克次体在两个物种中都表现出较高的丰度。柯克斯氏菌、阿森氏菌、螺旋体、方格氏菌和立克次体等几种母源遗传细菌数量丰富，其相对丰度因种类和性别的不同而不同，表明它们在这两个物种中的生物学作用。结论：现场采集的边缘毛滴虫和网纹毛滴虫的微生物区系受硬蜱系统发育和性别的影响。母系遗传细菌在这两个物种中都很丰富。这些发现对于了解扁虱与细菌的相互作用、生物学和扁虱的媒介能力具有重要价值。
Background:The important roles of microbial flora in tick biology and ecology have received much attention. Dermacentor marginatus and Dermacentor reticulatus are known vectors of various pathogens across Europe, including Slovakia. However, their bacterial microbiomes are poorly explored.Methods:In this study, bacterial microbiomes of field-collected D. marginatus and D. reticulatus from Slovakia were characterized using 16S rRNA high-throughput sequencing.Results:Different analyses demonstrated that the D. marginatus and D. reticulatus microbiomes differ in their diversity and taxonomic structures. Furthermore, species- and sex-specific bacteria were detected in the two species. A possible bacterial pathogen "Candidatus Rhabdochlamydia sp." was detected from D. marginatus males. Among the observed bacteria, Rickettsia showed high abundance in the two species. Several maternally inherited bacteria such as Coxiella, Arsenophonus, Spiroplasma, Francisella and Rickettsiella, were abundant, and their relative abundance varied depending on tick species and sex, suggesting their biological roles in the two species.Conclusions:The bacterial microbiomes of field-collected D. marginatus and D. reticulatus were shaped by tick phylogeny and sex. Maternally inherited bacteria were abundant in the two species. These findings are valuable for understanding tick-bacteria interactions, biology and vector competence of ticks.