Directed tagging of the Arabidopsis FATTY ACID ELONGATION1 (FAE1) gene with the maize transposon activator.
Directed tagging of the Arabidopsis FATTY ACID ELONGATION1 (FAE1) gene with the maize transposon activator.
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DOI：
10.1105/tpc.7.3.309
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发表时间：
1995-03
期刊：
影响因子：
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通讯作者：
D. W. James;E. Lim;J. Keller;Ingrid Plooy;E. Ralston;H. Dooner
中科院分区：
文献类型：
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作者：
D. W. James;E. Lim;J. Keller;Ingrid Plooy;E. Ralston;H. Dooner
拟南芥的脂肪酸延伸1（FAE 1）基因是种子中合成极长链脂肪酸所必需的。FAE 1基因的产物被推测为将脂肪酸的链长从C18延伸至C20和C22的缩合酶。我们在这里报告的FAE 1的定向转座子标记与玉米元件激活剂（Ac）的克隆。一个不稳定的fae 1突变体中分离的4号染色体上的FAE 1位点携带Ac连锁。共分离和回复突变分析表明，新的突变体是由Ac标记的。通过反向聚合酶链反应克隆Ac侧翼的DNA片段，并用于从由未成熟长角果制成的文库中分离FAE 1基因组克隆和cDNA克隆。FAE 1蛋白的预测氨基酸序列与其他缩合酶（查耳酮合酶、芪苷酶和β-酮脂酰-酰基载体蛋白合酶III）的氨基酸序列具有同源性，支持FAE 1是合酶或缩合酶的结构基因的观点。FAE 1在发育中的种子中表达，但在叶中不表达，正如从FAE 1突变对这些组织的脂肪酸组成的影响所预期的那样。
The FATTY ACID ELONGATION1 (FAE1) gene of Arabidopsis is required for the synthesis of very long chain fatty acids in the seed. The product of the FAE1 gene is presumed to be a condensing enzyme that extends the chain length of fatty acids from C18 to C20 and C22. We report here the cloning of FAE1 by directed transposon tagging with the maize element Activator (Ac). An unstable fae1 mutant was isolated in a line carrying Ac linked to the FAE1 locus on chromosome 4. Cosegregation and reversion analyses established that the new mutant was tagged by Ac. A DNA fragment flanking Ac was cloned by inverse polymerase chain reaction and used to isolate FAE1 genomic clones and a cDNA clone from a library made from immature siliques. The predicted amino acid sequence of the FAE1 protein shares homology with those of other condensing enzymes (chalcone synthase, stilbene synthases, and beta-ketoacyl-acyl carrier protein synthase III), supporting the notion that FAE1 is the structural gene for a synthase or condensing enzyme. FAE1 is expressed in developing seed, but not in leaves, as expected from the effect of the fae1 mutation on the fatty acid compositions of those tissues.