Francisella novicida can utilize Paramecium bursaria as its potential host
Francisella novicida can utilize Paramecium bursaria as its potential host
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新杀弗朗西斯菌可以利用草履虫作为其潜在宿主
DOI：
10.1111/1758-2229.13029
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发表时间：
2021
影响因子：
3.3
通讯作者：
Watarai Masahisa
中科院分区：
文献类型：
--
作者：
Watanabe Kenta;Motonaga Akane;Tachibana Masato;Shimizu Takashi;Watarai Masahisa
新杀弗朗西斯菌是一种兼性胞内病原菌，是兔热病的病原体。虽然有接触受污染的水引起感染的报告，但其自然宿主和环境生态仍不清楚。本研究探讨了绿草履虫（Paramecium bursariam）作为F. novicida。实验感染F. novicidesult在一个稳定的细胞内的关系在P。bursaria。这种共生的细胞内关系在其他弗朗西斯属和嗜肺军团菌的实验感染中没有观察到。我们发现F. novicididhad相似的行为，真核内共生体的P。bursaria，绿色藻类Chlorella，在内化过程中。此外，F. novicida只有在小球藻不存在的情况下才有可能。虽然我们研究了F. novicidaas作为细菌因子的候选者，我们发现它不参与与P. bursaria。这些结果表明P. bursarias可能是F. novicidaand可能是了解原生生物宿主与其共生体之间关系的有用工具。
Francisella novicidais a facultative intracellular pathogen and the causative agent of tularemia. Although cases of infection caused by exposure to contaminated water have been reported, its natural host and ecology in the environment remain unclear. In this study, we investigatedin vitrothe possibility thatParamecium bursariamay be a useful tool as a protist host model ofF. novicida. Experimental infection withF. novicidaresulted in a stable intracellular relationship withinP. bursaria. This symbiotic intracellular relationship was not observed in experimental infections with otherFrancisellaspecies andLegionella pneumophila. We found thatF. novicidashowed similar behaviour to that of the eukaryotic endosymbiont ofP. bursaria, the green algaeChlorella, in the internalization process. In addition, stable intracellular localization ofF. novicidawas possible only whenChlorellawas not present. Although we investigated the type VI secretion system ofF. novicidaas a candidate for the bacterial factor, we found that it was not involved in the establishment of an intracellular relationship withP. bursaria. These results suggested thatP. bursariais potentially a protist host model forF. novicidaand may be a useful tool for understanding the relationship between protist hosts and their symbionts.