The rate and character of spontaneous mutation in Thermus thermophilus
The rate and character of spontaneous mutation in Thermus thermophilus
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DOI：
10.1534/genetics.108.089086
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发表时间：
2008-09-01
期刊：
影响因子：
3.3
通讯作者：
Grogan, Dennis W.
中科院分区：
文献类型：
--
作者：
Mackwan, Reena R.;Carver, Geraldine T.;Grogan, Dennis W.
在两个染色体位点（pyrF和pyrE）上选择自发的功能丧失突变，使得极端嗜热细菌嗜热栖热菌首次实现了分子水平的复制保真度。两种不同的方法产生了相似的突变率，通过对独立突变体进行测序确定的突变谱揭示了分布在整个目标基因中的各种复制错误。从这些目标估计的基因组突变率（每次复制 0.00097 +/- 0.00052）低于嗜温微生物的相应估计值，主要是因为碱基取代率较低。然而，嗜热嗜热菌的自发突变率和谱与嗜热嗜酸古菌酸热硫化叶菌相似，尽管这两种嗜热微生物在其基因组中存在重要的分子差异。
Selection of spontaneous, loss-of-function mutations at two chromosomal loci (pyrF and pyrE) enabled the first molecular-level of replication fidelity in the extremely thermophilic bacterium Thermus thermophilus. Two different methods yielded similar mutation rates, mutational spectra determined by sequencing of independent mutants revealed a variety of replication errors distributed throughout the target genes. The genomic mutation rate estimated from these targets, 0.00097 +/- 0.00052 per replication, was lower than corresponding estimates from mesophilic microorganisms, primarily because of a low rate of base substitution.. However, both the rate and spectrum of spontaneous mutations T. thermophilus resembled those of the thermoacidophilic archaeon Sulfolobus acidocaldarius, despite important molecular differences between these two thermophiles mid their genomes.