EVIDENCE FOR TUBULAR MATING STRUCTURES INDUCED IN EACH MATING TYPE OF HETEROTHALLIC GONIUM PECTORALE (VOLVOCALES, CHLOROPHYTA)
EVIDENCE FOR TUBULAR MATING STRUCTURES INDUCED IN EACH MATING TYPE OF HETEROTHALLIC GONIUM PECTORALE (VOLVOCALES, CHLOROPHYTA)
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DOI：
10.1111/j.1529-8817.2012.01149.x
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发表时间：
2012-06-01
影响因子：
2.9
通讯作者：
Nozaki, Hisayoshi
中科院分区：
文献类型：
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作者：
Mogi, Yuko;Hamaji, Takashi;Nozaki, Hisayoshi
在不同交配型的异交、同配子的群落型耳蝠的培养上分别诱导出配子。观察交配类型正负(正负)的管状交配结构(TMS)，称为双侧交配乳头。在每种交配型约3周大的培养物中加入二丁酰环腺苷(DcAMP或db-cAMP)和3-异丁基-1-甲基黄嘌呤(IBMX)，可立即从细胞壁释放裸露的配子。正配子和负配子均在原生质体前部形成TMS。抗体染色显示肌动蛋白在两种交配类型的TMS(受精管)中均有积聚，与单细胞莱茵衣藻正配子的TMS(受精管)相同。在每种交配类型中用TMS诱导裸配子将有助于未来对集群草藻同配子的细胞生物学和进化研究。
Gametes were induced separately in cultures of each mating type of the heterothallic, isogamous colonial volvocalean Gonium pectorale O. F. Mull. to examine the tubular mating structure (TMS) of both mating types plus and minus (plus and minus), referred to as bilateral mating papillae. Addition of dibutyryl cyclic adenosine monophosphate (DcAMP or db-cAMP) and 3-isobutyl-1-methylxanthine (IBMX) to approximately 3-week-old cultures of each mating type induced immediate release of naked gametes from the cell walls. Both plus and minus gametes formed a TMS in the anterior region of the protoplasts. Accumulation of actin was visualized by antibody staining in the TMS of both mating types as occurs in the TMS (fertilization tubule) of the plus gametes of the unicellular volvocalean Chlamydomonas reinhardtii P. A. Dang. Induction of naked gametes with a TMS in each mating type will be useful for future cell biological and evolutionary studies of the isogametes of colonial volvocalean algae.