High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report.
High Serum Advanced Glycation End Products Are Associated with Decreased Insulin Secretion in Patients with Type 2 Diabetes: A Brief Report.
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DOI：
10.1155/2017/5139750
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发表时间：
2017
影响因子：
4.3
通讯作者：
Yamamoto K
中科院分区：
文献类型：
--
作者：
Okura T;Ueta E;Nakamura R;Fujioka Y;Sumi K;Matsumoto K;Shoji K;Matsuzawa K;Izawa S;Nomi Y;Mihara H;Otsuka Y;Kato M;Taniguchi SI;Yamamoto K
晚期糖基化终产物（AGEs）在2型糖尿病（T2DM）的病理生理中具有重要意义。它们直接导致动物和细胞培养模型中的胰岛素分泌缺陷，并可能促进非糖尿病受试者的胰岛素抵抗。我们建立了一种高灵敏度的液相色谱-串联质谱法测定人血清中AGEs。在这里，我们用这种方法来研究T2DM患者的AGEs与胰岛素分泌和抵抗的关系。我们的研究包括15名未服药的2型糖尿病患者和20名非糖尿病健康患者。测定AGE中羧乙基赖氨酸（CEL）、羧甲基赖氨酸（CML）和甲基乙二醛-氢咪唑酮（MG-H1）的含量。在饮食耐量试验中测量了这些参与者的血浆葡萄糖和胰岛素，并在血糖-高胰岛素钳夹期间测量了葡萄糖的处置率。T2DM患者CML和CEL水平明显高于非dm患者。T2DM患者CML与胰岛素分泌、HOMA-%B呈显著负相关，与胰岛素敏感性指数呈显著正相关。测定的AGEs与葡萄糖处理速率之间没有相关性。这些结果表明，AGE可能在2型糖尿病胰岛素分泌缺陷的发展或预测中发挥作用。
Advanced glycation end products (AGEs) are important in the pathophysiology of type 2 diabetes mellitus (T2DM). They directly cause insulin secretory defects in animal and cell culture models and may promote insulin resistance in nondiabetic subjects. We have developed a highly sensitive liquid chromatography-tandem mass spectrometry method for measuring AGEs in human serum. Here, we use this method to investigate the relationship between AGEs and insulin secretion and resistance in patients with T2DM. Our study involved 15 participants with T2DM not on medication and 20 nondiabetic healthy participants. We measured the AGE carboxyethyllysine (CEL), carboxymethyllysine (CML), and methyl-glyoxal-hydro-imidazolone (MG-H1). Plasma glucose and insulin were measured in these participants during a meal tolerance test, and the glucose disposal rate was measured during a euglycemic-hyperinsulinemic clamp. CML and CEL levels were significantly higher in T2DM than non-DM participants. CML showed a significant negative correlation with insulin secretion, HOMA-%B, and a significant positive correlation with the insulin sensitivity index in T2DM participants. There was no correlation between any of the AGEs measured and glucose disposal rate. These results suggest that AGE might play a role in the development or prediction of insulin secretory defects in type 2 diabetes.