Fluorescence-based isolation of bacterial genes expressed within host cells
Fluorescence-based isolation of bacterial genes expressed within host cells
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DOI：
10.1126/science.277.5334.2007
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发表时间：
1997-09-26
期刊：
影响因子：
56.9
通讯作者：
Falkow, S
中科院分区：
文献类型：
--
作者：
Valdivia, RH;Falkow, S
设计了一种选择策略来鉴定当细菌与其宿主细胞结合时优先表达的细菌基因。14个鼠伤寒沙门氏菌基因，这是在至少四个独立的调节回路的控制下，被确定为在宿主巨噬细胞中被选择性诱导。四个基因编码毒力因子，包括III型分泌器的组成部分。这种选择方法应普遍适用于鉴定病原生物体中与宿主细胞或组织结合后诱导的基因。
A selection strategy was devised to identify bacterial genes preferentially expressed when a bacterium associates with its host cell. Fourteen Salmonella typhimurium genes, which were under the control of at least four independent regulatory circuits, were identified to be selectively induced in host macrophages. Four genes encode virulence factors, including a component of a type III secretory apparatus. This selection methodology should be generally applicable to the identification of genes from pathogenic organisms that are induced upon association with host cells or tissues.