Quercetin attenuates carbon tetrachloride‐induced testicular damage in rats
Quercetin attenuates carbon tetrachloride‐induced testicular damage in rats
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DOI：
10.1111/and.12159
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发表时间：
2014-10
期刊：
影响因子：
2.4
通讯作者：
M. Sönmez;G. Türk;S. Çeribaşı;M. Çiftçi;A. Yüce;M. Guvenc;Ş. Kaya;M. Çay;M. Aksakal
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文献类型：
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作者：
M. Sönmez;G. Türk;S. Çeribaşı;M. Çiftçi;A. Yüce;M. Guvenc;Ş. Kaya;M. Çay;M. Aksakal
本研究旨在探讨槲皮素对四氯化碳（CCl4）诱导的雄性大鼠精子损伤、睾丸细胞凋亡和氧化应激的影响。第1组作为对照，第2组仅用槲皮素治疗，第3组仅用CCl4治疗，第4组接受CCl4+槲皮素治疗。所有给药均通过强饲法进行并维持10周。与对照组相比，CCl4 给药导致绝对和相对生殖器官重量、精子活力、浓度以及睾丸谷胱甘肽过氧化物酶 (GSH-Px) 和过氧化氢酶 (CAT) 活性显着下降，脂质过氧化 (LPO) 水平、异常精子率和睾丸凋亡细胞指数显着增加，以及一些组织病理学损伤。然而，与仅使用 CCl4 组相比，CCl4 与槲皮素一起给药在 LPO 水平、异常精子率、组织病理学病变程度和睾丸凋亡细胞指数方面具有统计学显着改善。此外，与仅 CCl4 组相比，第 4 组生殖器官的绝对和相对重量、精子活力和浓度、睾丸 GSH-Px 和 CAT 活性的改善在统计学上不显着。综上所述，槲皮素具有抗过氧化作用，口服槲皮素可通过降低LPO来减轻CCl4对男性生殖器官和细胞的损伤。
This study was conducted to investigate the effect of quercetin on carbon tetrachloride (CCl4)‐induced sperm damages, testicular apoptosis and oxidative stress in male rats. Group 1 served as control, group 2 was treated with only quercetin, group 3 was treated with only CCl4 and group 4 received CCl4 + quercetin. All administrations were performed by gavage and maintained for 10 weeks. CCl4 administration caused significant decreases in absolute and relative reproductive organ weights, sperm motility, concentration and testicular glutathione peroxidase (GSH‐Px) and catalase (CAT) activities, and significant increases in lipid peroxidation (LPO) level, abnormal sperm rate and testicular apoptotic cell index, along with some histopathological damages when compared to the control group. However, administration of CCl4 together with quercetin provided statistically significant improvements in LPO level, abnormal sperm rate, the degree of histopathological lesions and testicular apoptotic cell index when compared to only CCl4 group. In addition, improvements observed in absolute and relative weights of reproductive organs, sperm motility and concentration, and testicular GSH‐Px and CAT activities in group 4 were statistically insignificant when compared to only CCl4 group. In conclusion, quercetin has antiperoxidative effect, and its oral administration attenuates the CCl4‐induced some damages in male reproductive organs and cells by decreasing the LPO.