No apparent requirement for neuronal sodium channels in excitation-contraction coupling in rat ventricular myocytes
No apparent requirement for neuronal sodium channels in excitation-contraction coupling in rat ventricular myocytes
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DOI：
10.1161/01.res.0000209963.02720.70
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发表时间：
2006-03-17
影响因子：
20.1
通讯作者：
Orchard, CH
中科院分区：
文献类型：
--
作者：
Brette, F;Orchard, CH
心脏中的大多数Na通道由河豚毒素（TTX）抗性（K-D，2至6 μ mol/L）“心脏”Na（V）1.5亚型组成;然而，最近在几种心脏制备物中检测到TTX敏感性（K-D，1至25 nmol/L）“神经元”Na通道亚型。在本研究中，我们确定的功能亚细胞定位的Na通道亚型（根据其TTX的敏感性）在大鼠心室肌细胞通过记录I-Na在控制和去微管心肌细胞。我们发现，对照组中TTX敏感的I-Na（K-D，约为8.8 nmol/L）占总I-Na的14 ± 3%，
The majority of Na channels in the heart are composed of the tetrodotoxin (TTX)-resistant (K-D, 2 to 6 mu mol/L) "cardiac" Na(V)1.5 isoform; however, TTX-sensitive (K-D, 1 to 25 nmol/L) "neuronal" Na channel isoforms have recently been detected in several cardiac preparations. In the present study, we determined the functional subcellular localization of Na channel isoforms (according to their TTX sensitivity) in rat ventricular myocytes by recording I-Na in control and detubulated myocytes. We found that TTX-sensitive I-Na (K-D, approximate to 8.8 nmol/L) makes up 14 +/- 3% of total I-Na in control and