Co-Overexpression ofOsNAR2.1andOsNRT2.3aIncreased Agronomic Nitrogen Use Efficiency in Transgenic Rice Plants
Co-Overexpression ofOsNAR2.1andOsNRT2.3aIncreased Agronomic Nitrogen Use Efficiency in Transgenic Rice Plants
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DOI：
10.3389/fpls.2020.01245
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发表时间：
2020-08-12
影响因子：
5.6
通讯作者：
Xu, Guohua
中科院分区：
文献类型：
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作者：
Chen, Jingguang;Liu, Xiaoqin;Xu, Guohua
NO(3)(-)转运体在水稻氮素吸收和氮素利用效率中起着重要作用。我们以前的研究表明，水稻吸收硝酸盐的高亲和力系统是由一个双组分的NRT2/NAR2运输系统介导的。在本研究中，通过单独过表达OsNAR2.1、单独过表达OsNRT2.3a以及同时过表达OsNAR2.1和OsNRT2.3a，成功地获得了转基因植株。田间试验表明，转基因品系expressingp35S:OsNAR2.1orp35S:OsNAR2.1-p35S:OsNRT2.3aconstructs的籽粒产量分别比野生型增产14.1%和24.6%。农业氮素利用效率分别提高15.8%和28.6%。与对照相比，0.2 mM、2.5 mM(15)NO(3)(-)和1.25 mm(15)NH(4)(15)NO(3)对p35S：OsNAR2.1和p35S：OsNAR2.1-P35S：OsNRT2.3alines根系的~(15)N输入量增加了18.9%~27.8%，而差异不显著(P>0.05)，只有betweenp35S:OsNAR2.1andp35S:OsNAR2.1-p35S:OsNRT2.3alines；：OsNAR2.1-P35S：OsNRT2.3alines的地上部与根部的(15)N分配比显著增加。但是，p35S：NRT2.3转基因品系与对照相比，在氮素利用效率、根系N-15吸收和产量等方面没有显著差异。本研究表明，共表达OsNAR2.1和OsNRT2.3可以提高水稻产量和氮素利用效率。
The NO(3)(-)transporter plays an important role in rice nitrogen acquisition and nitrogen-use efficiency. Our previous studies have shown that the high affinity systems for nitrate uptake in rice is mediated by a two-component NRT2/NAR2 transport system. In this study, transgenic plants were successful developed by overexpression ofOsNAR2.1alone,OsNRT2.3aalone and co-overexpression ofOsNAR2.1andOsNRT2.3a. Our field experiments indicated that transgenic lines expressingp35S:OsNAR2.1orp35S:OsNAR2.1-p35S:OsNRT2.3aconstructs exhibited increased grain yields of approximately 14.1% and 24.6% compared with wild-type (cv. Wuyunjing 7, WT) plants, and the agricultural nitrogen use efficiency increased by 15.8% and 28.6%, respectively. Compared with WT, the(15)N influx in roots ofp35S:OsNAR2.1andp35S: OsNAR2.1-p35S:OsNRT2.3alines increased 18.9%-27.8% in response to 0.2 mM, 2.5 mM(15)NO(3)(-), and 1.25 mM(15)NH(4)(15)NO(3), while there was no significant difference betweenp35S:OsNAR2.1andp35S:OsNAR2.1-p35S:OsNRT2.3alines; only the(15)N distribution ratio of shoot to root forp35S:OsNAR2.1-p35S:OsNRT2.3alines increased significantly. However, there were no significant differences in nitrogen use efficiency,N-15 influx in roots and the yield between thep35S:NRT2.3atransgenic lines and WT. This study indicated that co-overexpression ofOsNAR2.1andOsNRT2.3acould increase rice yield and nitrogen use efficiency.