Construction of a novel DNA vaccine candidate targeting F gene of genotype VII Newcastle disease virus and chicken IL-18 delivered by Salmonella
Construction of a novel DNA vaccine candidate targeting F gene of genotype VII Newcastle disease virus and chicken IL-18 delivered by Salmonella
复制标题
构建针对基因 VII 新城疫病毒 F 基因和沙门氏菌传递的鸡 IL-18 的新型 DNA 候选疫苗
DOI：
10.1111/jam.14228
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发表时间：
2019-05-01
影响因子：
4
通讯作者：
Wang, C.
中科院分区：
文献类型：
--
作者：
Gao, X.;Xu, K.;Wang, C.
目的 基因VII型新城疫（ND）是家禽业中最具流行性和严重性的传染病之一。针对基因VII型新城疫病毒（NDV）的新型疫苗仍然难以研制。方法与结果 在本研究中，我们构建了调控延迟裂解的沙门氏菌菌株，其在真核CMV启动子下单独表达融合蛋白（F），或者在原核P - trc启动子下与鸡白细胞介素 - 18（chIL - 18）作为分子佐剂一起表达，分别命名为pYL1和pYL23。用重组菌株口服免疫在pYL1和pYL23免疫的鸡中均诱导出新城疫病毒特异性血清IgG抗体。chIL - 18的存在显著提高了免疫鸡的淋巴细胞增殖，以及血清中CD3(+)CD4(+)和CD3(+)CD8(+) T细胞的百分比，尽管不存在统计学上的显著差异。在强毒攻击后，pYL23免疫在感染后第10天提供了约80%的保护，相比之下，pYL1免疫提供了60%的保护，灭活疫苗组提供了100%的保护，这表明chIL - 18提供了增强的免疫反应，组织化学分析也证实了这一点。结论 重组裂解沙门氏菌载体DNA疫苗可为我们控制新城疫病毒感染提供一种新的潜在选择。研究的意义和影响 本研究利用调控延迟裂解的沙门氏菌载体设计了一种针对新城疫病毒的口服疫苗。
Aims Genotype VII Newcastle disease (ND) is one of the most epidemic and serious infectious diseases in the poultry industry. A novel vaccine targeting VII Newcastle disease virus (NDV) is still proving elusive. Methods and Results In this study, we constructed regulated delayed lysis Salmonella strains expressing either a fusion protein (F) alone under an eukaryotic CMV promoter or together with chicken IL-18 (chIL-18) as a molecular adjuvant under prokaryotic P-trc promoter, named pYL1 and pYL23 respectively. Oral immunization with recombinant strains induced NDV-specific serum IgG antibodies in both pYL1- and pYL23-immunized chickens. The presence of chIL-18 significantly increased lymphocyte proliferation in immunized chickens, as well as the percentages of CD3(+)CD4(+) and CD3(+)CD8(+) T cells in serum, even if a statistically significant difference did not exist. After a virulent challenge, pYL23 immunization provided about 80% protection at day 10 postinfection, compared with 60% of protection offered by pYL1 immunization and 100% protection in the inactivated vaccine group, indicating the enhanced immune response provided by chIL-18, which was also confirmed by histochemical analysis. Conclusions Recombinant lysis Salmonella-vectored DNA vaccine could provide us a novel potential option for controlling NDV infection. Significance and Impact of the Study This study took use of a regulated delayed lysis Salmonella vector for the design of an orally administrated vaccine against NDV.