Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation
Pancreas Preservation in Modified Histidine-lactobionate Solution Is Superior to That in University of Wisconsin Solution for Porcine Islet Isolation
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DOI：
10.1097/tp.0000000000003636
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发表时间：
2022-09-01
期刊：
影响因子：
6.2
通讯作者：
Noguchi，Hirofumi
中科院分区：
文献类型：
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作者：
Sakai-Yonaha，Mayuko;Miyagi-Shiohira，Chika;Noguchi，Hirofumi
背景：我们以前报道过，与威斯康星州（UW）保存法相比，改良的含细胞外型海藻糖的京都（MK）溶液（含有胰蛋白酶抑制剂（乌司他丁））显著提高了胰岛产量，后者是胰岛分离器官保存的金标准。在这项研究中，我们评估了除了UW或MK溶液之外，改性组氨酸乳糖酸盐（MHL）溶液的效率。与UW溶液相比，MHL溶液具有高钠低钾的组成，具有低粘度。方法：将猪胰腺保存在UW、MK或MHL液中，分离胰岛。优化数量（1500 IE）的分离的胰岛从各组，然后移植到链脲佐菌素诱导的diabetes.Results小鼠。胰岛产量纯化前和纯化后的MHL组比在UW组显着较高。相反，纯化前后的胰岛产量在MHL组和MK组之间没有显著差异。MHL液保存猪胰腺较UW液保存猪胰腺能更好地保存糖尿病小鼠胰岛移植的效果。MHL液与MK液作用相似，提示MHL液可作为MK液的替代物用于胰岛移植。
Background.We previously reported that modified extracellular-type trehalose-containing Kyoto (MK) solution, which contains a trypsin inhibitor (ulinastatin), significantly improved the islet yield compared with University of Wisconsin (UW) preservation, which is the gold standard for organ preservation for islet isolation. In this study, we evaluated the efficiency of a modified histidine-lactobionate (MHL) solution in addition to UW or MK solution. The MHL solution has a high sodium-low potassium composition with low viscosity compared with the UW solution. Moreover, similar to MK solution, MHL solution also contains ulinastatin.Methods.Porcine pancreata were preserved in UW, MK, or MHL solution, followed by islet isolation. An optimized number (1500 IE) of isolated islets from each group were then transplanted into streptozotocin-induced diabetic mice.Results.The islet yield before and after purification was significantly higher in the MHL group than in the UW group. On the contrary, the islet yield before and after purification was not significantly different between the MHL and MK groups. Preserving the porcine pancreata in MHL solution improved the outcome of islet transplantation in streptozotocin-induced diabetic mice compared with that in UW solution.Conclusions.Pancreas preservation with MHL solution preserves islet function better than UW solution. The effect of MHL solution is similar to that of MK solution, suggesting that MHL solution can be used as an alternative to MK solution for pancreatic islet transplantation.