Rhizobitoxine production and symbiotic compatibility of Bradyrhizobium from Asian and North American lineages of amphicarpaea.
Rhizobitoxine production and symbiotic compatibility of Bradyrhizobium from Asian and North American lineages of amphicarpaea.
复制标题
亚洲和北美双果类谱系的慢生根瘤菌的根瘤菌素生产和共生相容性。
DOI：
10.1139/w01-085
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发表时间：
2001
影响因子：
2.8
通讯作者：
N. Peters
中科院分区：
文献类型：
--
作者：
M. Parker;N. Peters
用来自北美豆科植物Amphalipaea bracteata（L.）弗恩（Phaseoleae-Glycininae）和近缘种Amphalipaea edgeworthii（Benth.）变种分析相对共生亲和性。Amphalopaea edgeworthii植株与从A. bracteata，但A. bracteata谱系与来自A.埃奇沃西。然而，一组A.当用日本慢生根瘤菌接种时，bracteata植物（谱系Ia）产生了与已知由根瘤毒素引起的类似的显著的叶片失绿。的β-胱硫醚酶抑制试验表明，显着量的根瘤毒素存在于这些日本慢生根瘤菌形成的根瘤。没有从A. bracteata在任何植物上诱导褪绿，并且β-胱硫醚酶测定未能在由这些分离物形成的根瘤中检测到根瘤毒素。研究了根瘤菌毒素在A.通过用埃氏慢生根瘤菌（Bradyrhizobiumelkanii）根瘤毒素产生菌株USDA 61和两种不能合成根瘤毒素的突变衍生物RX 17 E和RX 18 E的接种植物测试了埃奇沃氏菌的产毒能力。用野生型USDA 61接种的双头螺旋藻每株产生>150个结节，而用RX 17 E和RX 18 E接种的植物每株产生少于10个结节。因此，在A. edgeworthii似乎高度依赖于慢生根瘤菌菌株的根瘤毒素生产。
Reciprocal inoculations with Bradyrhizobium sp. isolates from the North American legume Amphicarpaea bracteata (L.) Fern. (Phaseoleae-Glycininae) and from a Japanese population of its close relative Amphicarpaea edgeworthii (Benth.) var. japonica were performed to analyze relative symbiotic compatibility. Amphicarpaea edgeworthii plants formed few or no nodules with any North American bradyrhizobial strains isolated from A. bracteata, but all A. bracteata lineages formed effective nitrogen-fixing nodules with Japanese Bradyrhizobium isolates from A. edgeworthii. However, one group of A. bracteata plants (lineage Ia) when inoculated with Japanese bradyrhizobia developed a striking leaf chlorosis similar to that known to be caused by rhizobitoxine. The beta-cystathionase inhibition assay demonstrated that significant amounts of rhizobitoxine were present in nodules formed by these Japanese bradyrhizobia. No North American bradyrhizobial isolate from A. bracteata induced chlorosis on any plants, and the beta-cystathionase assay failed to detect rhizobitoxine in nodules formed by these isolates. The role of rhizobitoxine in A. edgeworthii nodulation development was tested by inoculating plants with a Bradyrhizobium elkanii rhizobitoxine-producing strain, USDA 61, and two mutant derivatives, RX17E and RX18E, which are unable to synthesize rhizobitoxine. Amphicarpaea edgeworthii inoculated with wild-type USDA 61 developed >150 nodules per plant, while plants inoculated with RX17E and RX18E developed fewer than 10 nodules per plant. Thus, efficient nodule development in A. edgeworthii appears to be highly dependent on rhizobitoxine production by Bradyrhizobium strains.