Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
Applying Stereotactic Injection Technique to Study Genetic Effects on Animal Behaviors
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DOI：
10.3791/52653
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发表时间：
2015-05-01
影响因子：
1.2
通讯作者：
Mao, Yingwei
中科院分区：
文献类型：
--
作者：
McSweeney, Colleen;Mao, Yingwei
立体定向注射是一种将高滴度慢病毒递送到小鼠靶向脑区域的有用技术。慢病毒可以在相对集中的区域过度表达或敲低基因表达，而不会对脑组织造成重大损害。恢复后，可以对注射的小鼠进行各种行为任务测试，例如旷场测试（OFT）和强迫游泳测试（FST）。OFT旨在通过测量小鼠在新开放场地中心花费的时间来评估小鼠的运动和焦虑表型。与对照组相比，更焦虑的小鼠在新视野中心的时间明显更少。FST通过量化小鼠在放入一桶水中时不动的时间来评估抗抑郁表型。与对照动物相比，具有抗抑郁表型的小鼠将花费显著更少的时间不动。该方案的目的是使用立体定向注射慢病毒结合行为测试来评估遗传因素如何调节动物行为。
Stereotactic injection is a useful technique to deliver high titer lentiviruses to targeted brain areas in mice. Lentiviruses can either overexpress or knockdown gene expression in a relatively focused region without significant damage to the brain tissue. After recovery, the injected mouse can be tested on various behavioral tasks such as the Open Field Test (OFT) and the Forced Swim Test (FST). The OFT is designed to assess locomotion and the anxious phenotype in mice by measuring the amount of time that a mouse spends in the center of a novel open field. A more anxious mouse will spend significantly less time in the center of the novel field compared to controls. The FST assesses the anti-depressive phenotype by quantifying the amount of time that mice spend immobile when placed into a bucket of water. A mouse with an anti-depressive phenotype will spend significantly less time immobile compared to control animals. The goal of this protocol is to use the stereotactic injection of a lentivirus in conjunction with behavioral tests to assess how genetic factors modulate animal behaviors.