Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia
Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia
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DOI：
10.1111/j.1600-0897.2007.00566.x
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发表时间：
2008-03-01
影响因子：
3.6
通讯作者：
Pyles, Richard B.
中科院分区：
文献类型：
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作者：
Herbst-Kralovetz, Melissa M.;Quayle, Alison J.;Pyles, Richard B.
问题为了更好地了解先天性免疫反应性传播感染（STI）和适当的上皮细胞（EC）模型的阴道和宫颈粘膜，量化的Toll样受体（TLR）的表达，从人口的妇女是needed.Method的研究TLR基因表达进行了量化的主要和永生化的子宫颈内，子宫颈外，和阴道EC从多个捐助者。结果TLR 1 -3、5-9在各型EC中均有表达，其中TLR 2、3、5、6和CD 14表达最丰富。宫颈和阴道EC表达TLR 4。TLR 2、3、5和6的激动剂刺激引起细胞因子。TLR 4和7-9表达最低，并且不具有一致的生物活性。永生化EC一般建模的原代文化，但阐述显着降低cytokine levels.Conclusion TLR的表达模式是显着保守的整个研究人群和评估组织表明可预测的STI反应。结果支持谨慎使用永生化细胞生殖道建模。
Problem To better understand innate immune responses to sexually-transmitted infection (STI) and the appropriateness of epithelial cell (EC) models of the vaginal and cervical mucosa, quantified toll-like receptor (TLR) expression from a population of women is needed.Method of study TLR gene expression was quantified in primary and immortalized endocervical, ectocervical, and vaginal EC from multiple donors. TLR bioactivity was evaluated by cytokine elaboration.Results TLRI-3 and 5-9 were expressed in each EC type with TLR2, 3, 5, 6 and CD14 expressed most abundantly. TLR4 was expressed by endocervical and vaginal EC. Agonist stimulation of TLR2, 3, 5 and 6 elicited cytokines. TLR4 and 7-9 were minimally expressed and were not consistently bioactive. Immortalized EC generally modeled primary cultures but elaborated significantly reduced cytokine levels.Conclusion TLR expression patterns were remarkably conserved across the study population and evaluated tissues indicating a predictable responsiveness to STI. The results support cautious use of immortalized cells for genital tract modeling.