Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of hanwoo steers.
Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of hanwoo steers.
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DOI：
10.5483/bmbrep.2007.40.5.757
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发表时间：
2007-09
期刊：
影响因子：
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通讯作者：
Seung Hwan Lee;E. Park;Yong-Min Cho;Sung-Kon Kim;Jun-Heon Lee;J. Jeon;C. Lee;S. Im;Sung-Jong Oh;John Mitchell Thompson;D. Yoon
中科院分区：
文献类型：
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作者：
Seung Hwan Lee;E. Park;Yong-Min Cho;Sung-Kon Kim;Jun-Heon Lee;J. Jeon;C. Lee;S. Im;Sung-Jong Oh;John Mitchell Thompson;D. Yoon
牛肉大理石花纹的形成依赖于多个基因的协调表达。牛在12至27月龄之间显著增加背最长肌的肌内脂肪含量。本研究采用退火控制引物（ACP）-差异显示RT-PCR方法，对肥育早期（12月龄）和肥育后期（27月龄）肌内脂肪发育过程中可能参与肌内脂肪形成的差异表达基因（DEG）进行了鉴定。利用20个ACP随机引物，我们鉴定和测序了14个DEG。BLAST检索显示，在肥育后期，MDH、PI 4-K、铁蛋白、ICER、NID-2、WDNMI、telethonin、细丝蛋白和结蛋白（DES）基因的表达增加，而GAPD、COP VII、ACTA 1、CamK II和nebulin基因的表达减少。使用Gene Ontology数据库对14个已知基因进行功能分类的结果表明，MDH、GAPD和COP VII参与糖酵解和TCA循环等代谢途径，而telethonin、细丝蛋白、星云蛋白、结蛋白和ACTA 1参与肌肉收缩装置，PI 4-K、CamK II和ICER在生长因子或激素调节的信号转导途径中发挥作用。最后三个基因NID-2、WDNMI和铁蛋白参与铁转运和细胞外蛋白抑制。使用实时PCR确认7个基因（MDH、PI 4-K、铁蛋白、ICER、星云蛋白、WDNMI和telethonin）的表达模式。我们发现，新的转录抑制因子ICER基因在牛育肥后期和前脂肪细胞分化过程中高表达。这些信息可能有助于选择参与牛肌内脂肪发育的候选基因。
Marbling of cattle meat is dependent on the coordinated expression of multiple genes. Cattle dramatically increase their intramuscular fat content in the longissimus dorsi muscle between 12 and 27 months of age. We used the annealing control primer (ACP)-differential display RT-PCR method to identify differentially expressed genes (DEGs) that may participate in the development of intramuscular fat between early (12 months old) and late fattening stages (27 months old). Using 20 arbitrary ACP primers, we identified and sequenced 14 DEGs. BLAST searches revealed that expression of the MDH, PI4-K, ferritin, ICER, NID-2, WDNMI, telethonin, filamin, and desmin (DES) genes increased while that of GAPD, COP VII, ACTA1, CamK II, and nebulin decreased during the late fattening stage. The results of functional categorization using the Gene Ontology database for 14 known genes indicated that MDH, GAPD, and COP VII are involved in metabolic pathways such as glycolysis and the TCA cycle, whereas telethonin, filamin, nebulin, desmin, and ACTA1 contribute to the muscle contractile apparatus, and PI4-K, CamK II, and ICER have roles in signal transduction pathways regulated by growth factor or hormones. The final three genes, NID-2, WDNMI, and ferritin, are involved in iron transport and extracellular protein inhibition. The expression patterns were confirmed for seven genes (MDH, PI4-K, ferritin, ICER, nebulin, WDNMI, and telethonin) using real-time PCR. We found that the novel transcription repressor ICER gene was highly expressed in the late fattening stage and during bovine preadipocyte differentiation. This information may be helpful in selecting candidate genes that participate in intramuscular fat development in cattle.