Histological study in the brain of the reelin/Dab1-compound mutant mouse
Histological study in the brain of the reelin/Dab1-compound mutant mouse
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DOI：
10.1007/s12565-008-0009-7
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发表时间：
2009-02
影响因子：
1.2
通讯作者：
Tatsuro Yamamoto;T. Setsu;Ayako Okuyama-Yamamoto;T. Terashima
中科院分区：
文献类型：
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作者：
Tatsuro Yamamoto;T. Setsu;Ayako Okuyama-Yamamoto;T. Terashima
Reelin（Reln）缺陷型小鼠（reeler）和Dab 1缺陷型小鼠（yotari）是常染色体隐性突变型小鼠，其特征为小脑共济失调。以前，我们报道了Reelin和Dab 1蛋白在大脑皮层发育过程中的功能略有不同。为了详细分析Reelin和Dab 1蛋白的功能作用，我们尝试通过培育杂合子小鼠和yotarimice来产生areelin/Dab 1复合突变小鼠。我们检测了野生型（Reln+/+; Dab 1 +/+）、双杂合子（Relnrl/+; Dab 1 yot/+）、reeler（Relnrl/rl; Dab 1 +/+，Relnrl/rl; Dab 1 yot/+）、yotari（Reln+/+; Dab 1 yot/yot，Relnrl/+; Dab 1 yot/yot）和双化合物缺陷（Relnrl/rl; Dab 1 yot/yot）小鼠大脑和小脑皮质以及海马的细胞结构。尼氏染色显示reelin/Dab 1双杂合子小鼠（Relnrl/+; Dab 1 yot/+）未见异常。Threelin/Dab 1复合突变小鼠（Relnrl/rl; Dab 1 yot/yot）除reelerandyotarimice外，在大脑和小脑皮质以及海马中显示组织学异常。我们将HRP注射到这些动物的腰髓中，用不同的基因组合来检查皮质脊髓束（CST）神经元的分布模式。Threelin/Dab 1复合突变小鼠的CST神经元不局限于第V层，而是分散在整个运动皮层。定量和统计分析表明，Threelin/Dab 1复合突变小鼠的CST神经元的分布模式不同于其他任何一种突变小鼠。总之，尽管Reelin/Dab 1信号转导是发育期神经元中的主要级联，但其他信号转导级联（例如，Cdk-5/Dab 1通路）可能与Reelin/Dab 1信号转导以平行方式存在。
TheReelin(Reln)-deficient mouse (reeler) and theDab1-deficient mouse (yotari) are autosomal recessive mutant mice characterized by cerebellar ataxia. Previously, we reported that Reelin and Dab1 proteins have slightly different functions during the development of the cerebral cortex. To analyze the functional roles of Reelin and Dab1 proteins in detail, we attempted to generate areelin/Dab1compound-mutant mouse by breeding heterozygotereelerandyotarimice. We examined the cytoarchitecture of the cerebral and cerebellar cortices and the hippocampus of wild-type (Reln+/+;Dab1+/+), double-heterozygote (Relnrl/+;Dab1yot/+), reeler (Relnrl/rl;Dab1+/+,Relnrl/rl;Dab1yot/+),yotari(Reln+/+;Dab1yot/yot,Relnrl/+;Dab1yot/yot), and double-compound-deficient (Relnrl/rl;Dab1yot/yot) mice. Nissl staining demonstrated that no abnormality was recognized in the mice ofreelin/Dab1double-heterozygote (Relnrl/+;Dab1yot/+). Thereelin/Dab1-compound mutant mouse (Relnrl/rl;Dab1yot/yot) showed histological abnormalities in the cerebral and cerebellar cortices and the hippocampus, in addition to those ofreelerandyotarimice. We injected HRP into the lumbar cord of these animals with various gene compositions to examine the distribution pattern of corticospinal tract (CST) neurons. CST neurons of thereelin/Dab1-compound mutant mice were not confined to layer V, but scattered throughout the motor cortex. This quantitative and statistical analysis shows that the distribution pattern of CST neurons of thereelin/Dab1-compound mutant mouse differs from those of either of thereeleroryotaricounterparts. Taken together, although Reelin/Dab1 signal transduction is a primary cascade in neurons during developmental periods, other signaling cascades (e.g., the Cdk-5/Dab1 pathway) may lie in a parallel fashion to Reelin/Dab1 signal transduction.