SUPPRESSION OF A NONSENSE MUTATION IN MAMMALIAN-CELLS INVIVO BY THE AMINOGLYCOSIDE ANTIBIOTICS G-418 AND PAROMOMYCIN
SUPPRESSION OF A NONSENSE MUTATION IN MAMMALIAN-CELLS INVIVO BY THE AMINOGLYCOSIDE ANTIBIOTICS G-418 AND PAROMOMYCIN
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DOI：
10.1093/nar/13.17.6265
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
MOGG, AE
中科院分区：
文献类型：
--
作者：
BURKE, JF;MOGG, AE
大肠杆菌和酵母中的氨基糖苷类抗生素可以使核糖体在翻译过程中阅读终止密码子。这可能导致对无意义的突起的表型抑制。在此，我们首次证明了氨基糖苷类G-418和帕罗诺霉素在猴(COS-7)细胞体内具有相似的作用。用氨基糖苷类化合物抑制琥珀突变(TAG)可以将导入COS-7细胞的突变基因的活性恢复到野生型水平的近20%。
Aminoglycoside antibiotics in Escherichia coli and yeast can cause ribosomes to read through stop codons during translation. This can result in the phenotypic suppression of nonsense nutations. We show here for the first time that the aminoglycosides G–418 and paronomycin have similar effects in monkey (COS–7) cells in vivo. Suppression of an amber mutation (TAG) by aninoglycosides can restore the activity of a mutant gene transfected into COS–7 cells to almost 20% of wild type levels.