Nanobody: outstanding features for diagnostic and therapeutic applications
Nanobody: outstanding features for diagnostic and therapeutic applications
复制标题
DOI：
10.1007/s00216-019-01633-4
复制
发表时间：
2019-03-01
影响因子：
4.3
通讯作者：
Marco, M. -Pilar
中科院分区：
文献类型：
--
作者：
Salvador, J. -Pablo;Vilaplana, Lluisa;Marco, M. -Pilar
纳米抗体（Nbs）作为传统抗体（Abs）的替代品而出现，并在诊断和治疗等不同生物技术领域显示出巨大的潜力。不同的方法已经被描述用于Nbs的生产，这些方法面临着新的挑战，重点是提高收率，亲和力和降低生产成本。本文综述了这些挑战，以及不同类型分子（如蛋白质和小分子）检测的最新进展，并描述了它们在无创体内成像和体外检测中的潜在应用。此外，还概述了Nbs的独特特性，如内化、大小、热稳定性和化学稳定性、亲和力、血液清除率和标记程序。关于治疗应用，我们重点介绍了一些已经报道的关于Nbs用于治疗癌症、神经退行性疾病或传染病等几种疾病的例子。最后，讨论了未来的发展趋势、机遇和劣势。
Nanobodies (Nbs) have arisen as an alternative to conventional antibodies (Abs) and show great potential when used as tools in different biotechnology fields such as diagnostics and therapy. Different approaches have been described for the production of Nbs and these methods face new challenges focused on improving yield, affinity, and reducing production costs. This review summarizes these challenges, and also the latest advances in the detection of different kinds of molecules, such as proteins and small molecules, and describes their potential use for noninvasive in vivo imaging and for in vitro assays. Moreover, the unique properties of Nbs are outlined like internalization, size, thermal and chemical stability, affinity, blood clearance, and labeling procedures. Concerning therapeutic applications, we highlight some already reported examples about Nbs being used for the treatment of several diseases such as cancer, neurodegenerative or infectious diseases among others. Finally, future trends, opportunities, and disadvantages are also discussed.