二・三海産養殖魚のヘモグロビンの酸素平衡—赤潮によるハマチの大量へい死に関連して
二・三海産養殖魚のヘモグロビンの酸素平衡—赤潮によるハマチの大量へい死に関連して
复制标题
第二海和第三海养殖鱼类血红蛋白的氧平衡——与赤潮造成的黄尾鱼大量死亡的关系
DOI：
10.2331/suisan.47.403
复制
发表时间：
1981
影响因子：
0.4
通讯作者：
友利 岡市
中科院分区：
文献类型：
--
作者：
勝司 山口;克公 小川;登 武田;周久 橋本;友利 岡市
通过对养殖黄尾马鲛（Seriola quinqueradiata）、真鲷（Redsea brephrys major）和黑鲷（Mylio macrocephalus）血红蛋白氧解离曲线的测定，揭示了黄尾马鲛血红蛋白具有独特的氧平衡特性。在pH 7.4和6.7之间，它显示出很大的玻尔效应，Δlog P50/ΔpH=-1.3，但与两种鲷的血红蛋白相比，在pH 6.7以下几乎完全缺乏pH依赖性。引起养殖黄尾鱼大量死亡的赤潮浮游生物查顿氏藻的超声波处理对黄尾鱼血红蛋白的氧解离曲线影响不大。然而，黄尾鱼的鳃和血液的pH值下降到6.7。根据这些结果和其他工作者以前的研究结果，可以说，赤潮浮游生物中的某种刺激性物质会引发鱼类的剧烈游动，这不可避免地导致肌肉中乳酸的积累和血液中pH值的下降，导致鱼类即使在氧气饱和的海水中也会窒息。
The measurement of oxygen dissociation curves of hemoglobins of cultured yellowtail Seriola quinqueradiata, red sea bream Chrysophrys major, and black sea bream Mylio macrocephalus revealed that yellowtail hemoglobin has a unique property in oxygen equilibria. It showed a large Bohr effect, Δlog P50/ΔpH=-1.3, between pH 7.4 and 6.7 but lacked the pH dependence almost entirely below pH 6.7 in contrast to the hemoglobins of the two breams. The sonicate of the red tide plankton, Chattonella sp. which causes the mass mortality of cultured yellowtail, had little influence on the oxygen dissociation curves of yellowtail hemoglobin. However, the pH values of the gills and blood of the yellowtails kept in the red tide water fell down to 6.7. From these results and previous findings of other workers, it may be said that some irritating substance from the red tide plankton should trigger a violent swim of the fish, which inevitably induces the accumulation of lactic acid in the muscle and the drop of pH in the blood, leading the fish to asphyxiation even in the oxygen saturated sea water.