THE DIVERGENCE BETWEEN 2 ONCOGENIC HERPESVIRUS-SAIMIRI STRAINS IN A GENOMIC REGION RELATED TO THE TRANSFORMING PHENOTYPE
THE DIVERGENCE BETWEEN 2 ONCOGENIC HERPESVIRUS-SAIMIRI STRAINS IN A GENOMIC REGION RELATED TO THE TRANSFORMING PHENOTYPE
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DOI：
10.1016/0042-6822(90)90020-r
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发表时间：
1990-06-01
期刊：
影响因子：
3.7
通讯作者：
FLECKENSTEIN, B
中科院分区：
文献类型：
--
作者：
BIESINGER, B;TRIMBLE, JJ;FLECKENSTEIN, B
根据猴猴疱疹病毒独特序列DNA左端的序列差异，可以将猴猴疱疹病毒株至少分为A、B、C三个亚群。A亚群和C亚群菌株具有高致癌性，在体外容易将类人猿t淋巴细胞转化为不依赖白细胞介素-2的生长，而B亚群菌株则没有。一种猿猴亚群A菌株的左端阅读框先前被证明与致癌表型和体外转化潜力相关；推导出的多肽命名为STP-A。此外，该区域还包含二氢叶酸还原酶（DHFR）的开放阅读框（ORF）和5种病毒特异性U rna （HSURs）的基因。我们现在通过对C亚群菌株相应区域的序列分析表明，DHFR和HSUR基因在两个病毒亚群中都存在；然而，在该C亚群病毒中未发现与STP-A阅读框同源的序列。在与STP-A相似的位置和取向上，发现了两个orf，用于共享假定的跨膜结构域的肽。其中一种基因编码了一种类似胶原蛋白的重复肽。除了与STP-A缺乏相似性外，这两个阅读框也没有显示出与已知癌基因的任何相似性。在猴猴独特的L-和重复的H-DNA的左交界处的序列组织表明频繁的重组事件，可能加速了病毒对外来基因的吸收。
Herpesvirus saimiri strains can be divided into at least three subgroups (A, B, C) based on sequence divergence at the left end of viral unique sequence DNA. Strains of subgroups A and C are highly oncogenic and readily transform simian T-lymphocyte in vitro to interleukin-2 independent growth, while subgroup B strains do not. A left terminal reading frame of a H. saimiri subgroup A strain was shown previously to correlate with the oncogenic phenotype and in vitro transforming potential; the deduced polypeptide was termed STP-A. Furthermore, this same region contains an open reading frame (ORF) for dihydrofolate reductase (DHFR) and genes for five virus-specific U RNAs (HSURs). We now shown by sequence analysis of the corresponding region in a subgroup C strain that DHFR and HSUR genes are present in both virus subgroups; however, no sequence homologous to the STP-A reading frame was found in this subgroup C virus. At a position and orientation similar to STP-A, two ORFs were found for peptides sharing a putative transmembrane domain. One of them encodes a peptide with collagen-like repetitions. In addition to the lack of similarity to STP-A, these two reading frames also did not show any similarity to known oncogenes. The organization of sequences at the left junction of unique L- and repetitive H-DNA of H. saimiri suggests frequent recombinational events, possibly accelerating the uptake of foreign genes by the virus.