An in-house constructed dual channel confocal fluorescence microscope for biomolecular imaging
An in-house constructed dual channel confocal fluorescence microscope for biomolecular imaging
复制标题
用于生物分子成像的内部构建的双通道共焦荧光显微镜
作者：
P. K. Shakhi;M. M. Bijeesh;Geetha K. Varier;P. Nandakumar
共聚焦荧光显微镜是生命科学研究中必不可少的活细胞成像工具。生物医学研究领域的一些实验研究需要专用的共聚焦荧光显微镜。然而，商业共焦显微镜是昂贵的，许多个别实验室，他们往往有一个不灵活的设计，不服从用户所需的修改。在这里，我们报告的设计，开发和校准的一个具有成本效益的双通道共聚焦荧光显微镜，可以同时捕获两个生物事件。该显微镜成功地用于成像和研究同时发生的分子穿过核膜的主动和被动运输。在延时成像模式中，监测沿着直径为20 nm的金纳米颗粒的主动运输的FITC标记的葡聚糖分子的被动扩散。 在毛地黄皂苷透化的HeLa细胞中进行的实验表明，主动和被动核转运途径共存。
The confocal fluorescence microscope is an essential live cell imaging tool in bioscience research. Several experimental investigations in the field of biomedical research require a dedicated confocal fluorescence microscope. However, commercial confocal microscopes are prohibitively expensive for many individual laboratories and they often have an inflexible design not amenable to user desired modifications. Here we report on the design, development, and calibration of a cost-effective dual channel confocal fluorescence microscope that can capture two biological events simultaneously. The microscope is successfully employed to image and study the simultaneously occurring active and passive transport of molecules across the nuclear membrane. Passive diffusion of FITC labelled dextran molecules are monitored along with the active transport of gold nanoparticles of diameter 20 nm in the time-lapse imaging mode. The experiments carried out in digitonin permeabilized HeLa cells indicate that both active and passive nuclear transport pathways coexist together.
影响因子：
4
作者：
Callamaras, N;Parker, I
通讯作者：
Parker, I