A gene cluster involved in the biosynthesis of vanchrobactin, a chromosome-encoded siderophore produced by Vibrio anguillarum
A gene cluster involved in the biosynthesis of vanchrobactin, a chromosome-encoded siderophore produced by Vibrio anguillarum
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DOI：
10.1099/mic.0.29298-0
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发表时间：
2006-12-01
期刊：
影响因子：
2.8
通讯作者：
Lemos, Manuel L.
中科院分区：
文献类型：
--
作者：
Balado, Miguel;Osorio, Carlos R.;Lemos, Manuel L.
鳗弧菌血清型O2菌株产生名为vanchrobactin的儿茶酚铁载体，其已被鉴定为N-[N '-（2，3-二羟基苯甲酰基）-乙酰基]-丝氨酸。这项工作描述了一个染色体区域，该区域含有vanchrobactin生物合成所需的遗传决定因素。作者已经鉴定了参与2，3-二羟基苯甲酸（DHBA）生物合成（vabA、vabB和vabC）和活化（vabE）的基因，以及编码非核糖体肽合成酶的基因（vabF），该合成酶参与铁载体组分的组装。还描述了一个推定的vanchrobactin出口（vabS）和铁载体酯酶（vabH）编码基因的鉴定和表征。在vabA，vabB，vabC，vabE，vabF和vabH的框内缺失突变体在铁限制的条件下生长受损，并且通过Chrome天青-S和交叉喂养试验对培养物上清液的分析显示在任何vabABCEF突变体中几乎不产生铁载体。此外，如通过化学和生物学分析所评估的，vabA、vabB和vabC的缺失突变废除了DHBA的产生。每个突变体与反式提供的相应基因的互补证实了该基因簇参与鳗弧菌菌株RV 22中DHBA和万古菌素的生物合成。基于化学和遗传数据，并在其他儿茶酚铁载体公布的模型，vanchrobactin生物合成的模型提出。
Vibrio anguillarum serotype O2 strains produce a catechol siderophore named vanchrobactin, which has been identified as N-[N'-(2,3-dihydroxybenzoyl)-arginyl]-serine. This work describes a chromosomal region that harbours the genetic determinants necessary for the biosynthesis of vanchrobactin. The authors have identified the genes involved in 2,3-dihydroxybenzoic acid (DHBA) biosynthesis (vabA, vabB and vabC) and activation (vabE), and a gene (vabF) encoding a non-ribosomal peptide synthetase, which is putatively involved in the assembly of the siderophore components. Also described are the identification and characterization of genes encoding a putative vanchrobactin exporter (vabS) and a siderophore esterase (vabH). In-frame deletion mutants in vabA, vabB, vabC, vabE, vabF and vabH were impaired for growth under conditions of iron limitation, and the analysis of culture supernatants by chrome azurol-S and cross-feeding assays showed almost no production of siderophores in any of the vabABCEF mutants. In addition, deletion mutations of vabA, vabB and vabC abolished production of DHBA, as assessed by chemical and biological analyses. Complementation of each mutant with the corresponding gene provided in trans confirmed the involvement of this gene cluster in the biosynthesis of DHBA and vanchrobactin in V. anguillarum strain RV22. Based on chemical and genetic data, and on published models for other catechol siderophores, a model for vanchrobactin biosynthesis is proposed.