Effects of acupuncture on vasopressin-induced emesis in conscious dogs
Effects of acupuncture on vasopressin-induced emesis in conscious dogs
复制标题
DOI：
10.1152/ajpregu.00344.2004
复制
发表时间：
2005-02-01
影响因子：
2.8
通讯作者：
Takahashi, T
中科院分区：
文献类型：
--
作者：
Tatewaki, M;Strickland, C;Takahashi, T
虽然针灸具有显著的临床疗效，但其作用机制尚不清楚。加压素是一种垂体后叶激素，与人类和狗的恶心和呕吐有关。为探讨针刺对抗利尿激素诱导的呕吐的止吐作用，记录清醒犬胃十二指肠运动活动及干呕、呕吐频率。在7只狗中，4个力传感器被植入胃体、胃窦、幽门和十二指肠的浆膜表面。在整个实验过程中连续监测胃十二指肠运动。血管加压素静脉滴注剂量为0.1 u.g g（-1）。Min(-1) 20分钟。在加压素输注前、中、后分别对心包-6 （PC6）、膀胱-21 （BL21）、胃-36 （ST36）进行电针（EA, 1-30 Hz）。为了研究阿片通路是否参与EA诱导的止吐作用，在EA和加压素输注之前、期间和之后分别给予纳洛酮（一种中枢和外周阿片受体拮抗剂）或纳洛酮甲氧基胺（一种外周阿片受体拮抗剂）。静脉输注抗利尿激素引起所有犬的干呕和呕吐。反胃收缩发生在干呕前。PC6时的EA （10 Hz）显著减少了干呕和呕吐的发作次数。PC6处EA也抑制逆行蠕动收缩。相比之下，BL21或ST36的EA无止吐作用。纳洛酮对EA的止吐作用有抑制作用，而纳洛酮对EA的止吐作用没有抑制作用。提示针灸的止吐作用是通过中枢阿片通路介导的。
Although acupuncture has a significant clinical benefit, the mechanism of acupuncture remains unclear. Vasopressin, a posterior pituitary hormone, is involved in nausea and vomiting in humans and dogs. To investigate the antiemetic effects of acupuncture on vasopressin-induced emesis, gastroduodenal motor activity and the frequency of retching and vomiting were simultaneously recorded in conscious dogs. In seven dogs, four force transducers were implanted on the serosal surfaces of the gastric body, antrum, pylorus, and duodenum. Gastroduodenal motility was continuously monitored throughout the experiment. Vasopressin was intravenously infused at a dose of 0.1 U.kg(-1).min(-1) for 20 min. Electroacupuncture ( EA, 1-30 Hz) at pericardium-6 (PC6), bladder-21 (BL21), or stomach-36 (ST36) was performed before, during, and after the vasopressin infusion. To investigate whether the opioid pathway is involved in EA-induced antiemetic effects, naloxone ( a central and peripheral opioid receptor antagonist) or naloxone methiodide ( a peripheral opioid receptor antagonist) was administered before, during, and after EA and vasopressin infusion. Intravenous infusion of vasopressin induced retching and vomiting in all dogs tested. Retrograde peristaltic contractions occurred before the onset of retching and vomiting. EA (10 Hz) at PC6 significantly reduced the number of episodes of retching and vomiting. EA at PC6 also suppressed retrograde peristaltic contractions. In contrast, EA at BL21 or ST36 had no antiemetic effects. The antiemetic effect of EA was abolished by pretreatment with naloxone but not naloxone methiodide. It is suggested that the antiemetic effect of acupuncture is mediated via the central opioid pathway.