Effect of electrical stimulation of nucleus accumbens with low, median and high currents intensities on conditioned place preference induced by morphine in rats.
Effect of electrical stimulation of nucleus accumbens with low, median and high currents intensities on conditioned place preference induced by morphine in rats.
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DOI：
10.4103/2277-9175.124643
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发表时间：
2014
影响因子：
1
通讯作者：
Alaei H
中科院分区：
文献类型：
--
作者：
Radahmadi M;Ramshini E;Hosseini N;Karimi S;Alaei H
一些研究人员指出，电或化学刺激对大脑不同部位的影响及其对动物行为的影响。此外，众所周知，吸毒与记忆和动机系统的功能障碍有关。本研究旨在观察不同电流强度电刺激伏核(NAC)对吗啡条件性位置偏爱的影响。雄性Wistar大鼠随机分为实验组(n=8)。我们观察了不同电流强度(低：15μA、中值：50μA和高：100μA)电刺激NAc和无效剂量吗啡(0.5 mg/kg)和有效剂量吗啡(5 mg/kg)对雄性大鼠吗啡诱导的位置条件反射获得和表达的影响。皮下注射吗啡(2.5和5 mg/kg，P<0.0 5和P<0.0 5，P<0.0 1)。此外，我们的结果表明，电刺激NAc(100μA)可抑制吗啡诱导的CPP。这揭示了吗啡给药导致条件反射过程中学习和记忆形成的障碍。大电流强度(100μA)可能具有逆转吗啡诱导的慢性帕金森病大鼠NAc中多巴胺及其代谢物含量增加的效应。此外，高电流强度结合无效剂量的吗啡(0.5 mg/kg)可增加吗啡诱导的CPP的概率，这是通过证明奖赏系统实现的。
Some investigators indicated the effect of electrical or chemical stimulation on different parts of the brain and its effect on animal's behaviors. Furthermore, drug addiction is known to be associated with dysfunction of memory and motivational systems. In this study, we aimed to evaluate the effect of electrical stimulation of nucleus accumbens (NAc) with different currents intensities on conditioned place preference (CPP) induced by morphine. Male Wistar rats were randomly divided for experimental groups (n = 8). We investigated the influence of electrical stimulation with different current intensities (low: 15 μA, median: 50 μA and high: 100 μA) on NAc with ineffective and effective dose of morphine (0.5 and 5 mg/kg, respectively) on acquisition and expression of morphine-induced place conditioning in male rats. The doses of subcutaneous administration morphine (2.5 and 5 mg/kg, P < 0.05 and P < 0.001; respectively) induced CPP compared with saline group. Furthermore, our findings are showed that electrical stimulation (100 μA) of NAc suppressed morphine-induced CPP. It revealed impairment of learning and memory formation in conditioning process due to morphine administration. It is possible that high current intensity (100 μA) had an accompanied effect by a reversal of the increased tissue contents of dopamine and its metabolites in the NAc of morphine-induced CPP rats. Furthermore, high current intensity in combination with ineffective dose of morphine (0.5 mg/kg) increased morphine-induced CPP probability via the prove reward system.