A METHOD FOR ISOLATION OF INTACT, TRANSLATIONALLY ACTIVE RIBONUCLEIC-ACID
A METHOD FOR ISOLATION OF INTACT, TRANSLATIONALLY ACTIVE RIBONUCLEIC-ACID
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DOI：
10.1089/dna.1983.2.329
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
BAXTER, JD
中科院分区：
文献类型：
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作者：
CATHALA, G;SAVOURET, JF;BAXTER, JD
本文提出了一种分离大的、具有抑制活性的RNA的方法。该方法包括将细胞或组织在5mGuanidine monothiocyanate中匀浆，然后用4mLiCl从胍中直接沉淀RNA。描述了用于组织培养细胞、酵母、组织或分离的细胞核的修饰。该程序的优点包括速度，简单，避免超离心，其适用于大量的小样品。该程序产生高达10 kb的大mRNA前体和翻译非常好的mRNA种类。然而，小（<300个核苷酸）RNA种类以低的产率回收。
A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5mguanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4mLiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (<300 nucleotides) RNA species are recovered with a poor yield.