Conserved alternative splicing patterns and splicing signals in the Drosophila sodium channel gene para.
Conserved alternative splicing patterns and splicing signals in the Drosophila sodium channel gene para.
复制标题
果蝇钠通道基因中保守的选择性剪接模式和剪接信号。
DOI：
10.1093/genetics/141.1.203
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发表时间：
1995
期刊：
影响因子：
3.3
通讯作者：
Ganetzky,B
中科院分区：
文献类型：
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作者：
Thackeray,JR;Ganetzky,B
我们克隆了与果蝇virilis para（编码钠通道α亚基的基因）同源的基因组DNA，并从胚胎和成虫中获得了许多部分cDNA克隆。 Para 蛋白得到了很好的保守，除了一个新的可选外显子之外，D. melanogaster 中六个不同剪接位点的可选元件也存在于 D. virilis 中。在 D. virilis 中观察到的 31 种不同剪接类型中，在 D. melanogaster 中观察到的阶段特异性可变剪接模式也是保守的。基因组 DNA 序列的比较揭示了替代使用的外显子序列和组成型使用的外显子序列之间存在三个差异。十六个短片段（10-75 bp）是唯一可识别的保守内含子序列，与替代使用的剪接位点不成比例地相关。与组成型外显子元件相比，在替代中发现沉默位点取代的频率要低得多，并且在较少选择的替代剪接点处，与果蝇剪接位点一致性的匹配程度往往较低。这项研究表明，副产物的发育调节变异性是高度保守的，因此可能具有功能意义，并表明多种不同的序列依赖性机制可能调节这种选择性剪接模式。
We cloned genomic DNA corresponding to the Drosophila virilis homologue of para, a gene encoding a sodium channel alpha-subunit, and obtained many partial cDNA clones from embryos and adults. Para protein has been well conserved, and the optional elements at six different sites of alternative splicing in D. melanogaster are present in D. virilis, in addition to one new optional exon. Among 31 different splice-types observed in D. virilis, the stage-specific pattern of alternative splicing seen in D. melanogaster is also conserved. Comparison of genomic DNA sequence revealed three aspects that vary between alternatively and constitutively used exon sequences. Sixteen short blocks (10-75 bp), the only recognizably conserved intron sequence, were disproportionately associated with alternatively used splice sites. Silent site substitutions were found much less frequently in alternative than constitutive exon elements, and the degree of match to the Drosophila splice site consensus tended to be lower at less frequently selected alternative splice junctions. This study shows that the developmentally regulated variability of para products is highly conserved and therefore likely to be of functional significance and suggests that a variety of different sequence-dependent mechanisms may regulate this pattern of alternative splicing.
DOI：
--
发表时间：
1994-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Lagrutta;Ke-Zhong Shen;R. North;John P. Adelman
通讯作者：
A. Lagrutta;Ke-Zhong Shen;R. North;John P. Adelman