Enzyme Immobilization in Polymerized Ionic Liquids-based Hydrogels for Active and Reusable Biocatalysts
Enzyme Immobilization in Polymerized Ionic Liquids-based Hydrogels for Active and Reusable Biocatalysts
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DOI：
10.1055/s-0037-1610144
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发表时间：
2018-04-01
期刊：
影响因子：
2.5
通讯作者：
Grosseheilmann, J.
中科院分区：
文献类型：
--
作者：
Grollmisch, A.;Kragl, U.;Grosseheilmann, J.
在聚合离子液体（PIL）中固定化酶有望成为一种简单回收和重复使用催化剂的通用工具。在这项研究中，CalB的粗提取物被封装在聚（VEImBr）和评估溶剂，温度，酶的量，浸出行为，和可重复使用的外消旋-1-苯基乙醇与乙酸乙烯酯的动力学拆分的例子。与非固定化酶相比，该固定化方法增加了CalB粗提取物的酶活性。该方法合成的目标产物具有较高的对映体过量（ee），且在实验中没有观察到活性酶的流失。将固定方法与Novozyme 435和Lipozyme RM IM（作为市售固定物）进行比较。非极性溶剂，包括正庚烷和正十二烷，被证明是最好的反应溶剂，表现出几乎完全的转化率和高的催化活性。包封的脂肪酶很容易从反应混合物中回收，并重复使用10个循环。
Enzyme immobilization in polymerized ionic liquids (PILs) promises to be a versatile tool for simple recovery and reuse of catalysts. In this study, a raw extract of CalB was encapsulated in poly(VEImBr) and assessed with respect to solvent, temperature, amount of enzyme, leaching behavior, and reusability on the example of the kinetic resolution of rac-1-phenylethanol with vinyl acetate. This immobilization method increased the enzyme activity of the CalB raw extract in comparison to the non- immobilized enzyme. The desired product was synthesized with high enantiomeric excess (ee) and no leaching of active enzyme was observed in the experiments. The immobilization method was compared to Novozyme 435 and Lipozyme RM IM, as commercially available immobilisates. Nonpolar solvents, including n- heptane and n- dodecane, proved to be the best reaction solvents, showing nearly full conversion and high catalytic activities. The encapsulated lipase was easily recovered from the reaction mixture and reused for ten cycles.