A Population Genetics Perspective on Geminivirus Infection
A Population Genetics Perspective on Geminivirus Infection
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DOI：
10.1128/jvi.01956-15
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发表时间：
2015-12-01
影响因子：
5.4
通讯作者：
Jeske, Holger
中科院分区：
文献类型：
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作者：
Weigel, Karin;Pohl, Jens O.;Jeske, Holger
在烟草本氏植株的早期系统感染过程中，记录了一种二分双生病毒，Abutilon花叶病毒的两种DNA组分的选择性积累。使用DNA A或DNA B特异性杂交诊断纯化的细胞核中的病毒DNA，以通过双色染色单独或同时检测这些单独的基因组组分。虽然这种病毒需要这两种成分的症状感染，DNA A单独运输到上部叶片，在那里它被输入到韧皮部细胞核和自主复制。DNA A和DNA B的共感染揭示了两种分子的独立传播，这导致了DNA A和DNA A/B感染的细胞核的随机分布。将相应频率的群体遗传学评价与模型计算进行比较。这阐明了一个令人惊讶的简单的关系之间的初始频率的病毒DNA成分和易感细胞的数量在早期全身infection.IMPORTANCEFor二分begomoviruses的过程中，DNA B-独立的长距离传播的DNA A已经被描述过，但它从来没有被证明是否病毒DNA A单独侵入细胞核的全身组织和复制。这是第一次被证明。在DNA A和DNA B感染期间，在早期阶段可以识别出DNA A的类似孤立扩散。我们描述了一个群体遗传学模型，该模型描述了易感细胞DNA A和DNA B的命中概率如何决定感染过程中任一基因组组分的相对频率。
The selective accumulation of both DNA components of a bipartite geminivirus, Abutilon mosaic virus, was recorded during early systemic infection of Nicotiana benthamiana plants. Purified nuclei were diagnosed for viral DNA using hybridization specific for DNA A or DNA B to detect these individual genome components either alone or both simultaneously by dual-color staining. Although this virus needs both components for symptomatic infection, DNA A alone was transported to upper leaves, where it was imported into phloem nuclei and replicated autonomously. The coinfection with DNA A and DNA B revealed an independent spread of both molecules, which resulted in a stochastic distribution of DNA A-and DNA A/B-infected nuclei. A population genetics evaluation of the respective frequencies was compared to a model computation. This elucidated a surprisingly simple relationship between the initial frequencies of the viral DNA components and the number of susceptible cells during the course of early systemic infection.IMPORTANCEFor bipartite begomoviruses, DNA B-independent long-distance spread of DNA A has been described before, but it has never been shown whether viral DNA A alone invades nuclei of systemic tissues and replicates therein. This is demonstrated now for the first time. During infection with DNA A and DNA B, a similar solitary spread of DNA A can be recognized at early stages. We describe a population genetics model of how the hit probabilities of DNA A and DNA B for susceptible cells determine the relative frequencies of either genome component during the course of infection.