Dual-enzyme cascade--an amplified method for the detection of alkaline phosphatase.
Dual-enzyme cascade--an amplified method for the detection of alkaline phosphatase.
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DOI：
10.1016/0003-2697(89)90120-6
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发表时间：
1989-06
影响因子：
2.9
通讯作者：
P. Mize;R. Hoke;C. Linn;J. Reardon;T. Schulte
中科院分区：
文献类型：
--
作者：
P. Mize;R. Hoke;C. Linn;J. Reardon;T. Schulte
描述了一种使用双酶级联快速灵敏地检测碱性磷酸酶的方法。一种掩蔽抑制剂，4-(3-氧代-4,4,4-三氟丁基)苯基磷酸酯，在碱性磷酸酶的作用下被去磷酸化。所得化合物 1,1,1-三氟-4-(4-羟基苯基)-丁-2-酮，可作为第二种酶（肝羧酸酯酶）的有效抑制剂。残余酯酶活性的测定提供了原始磷酸酶浓度的高度灵敏的指示。这种双酶级联的灵敏度比用磷酸对硝基苯酯直接检测磷酸酶活性所观察到的灵敏度高大约 125 倍。
A method in which a two-enzyme cascade is used for rapid and sensitive detection of alkaline phosphatase is described. A masked inhibitor, 4-(3-oxo-4,4,4-trifluorobutyl)phenyl phosphate, is dephosphorylated by the action of alkaline phosphatase. The resulting compound, 1,1,1-trifluoro-4-(4-hydroxyphenyl)-butan-2-one, acts as a potent inhibitor of the second enzyme, a liver carboxylesterase. A determination of the residual esterase activity provides a highly sensitive indication of the original phosphatase concentration. The sensitivity of this dual-enzyme cascade is approximately 125-fold greater than that observed for the direct detection of phosphatase activity with p-nitrophenyl phosphate.