Protective Effect of Flos Puerariae Extract Following Acute Alcohol Intoxication in Mice
Protective Effect of Flos Puerariae Extract Following Acute Alcohol Intoxication in Mice
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DOI：
10.1111/acer.12437
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发表时间：
2014-07-01
影响因子：
3.2
通讯作者：
Liu, Chao
中科院分区：
文献类型：
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作者：
Chen, Xiao;Cai, Fei;Liu, Chao
背景资料：方法：采用乙醇（EtOH）8 g/kg灌胃建立小鼠急性乙醇中毒模型，观察红花提取物（FPE）对乙醇代谢、肝损伤及记忆障碍的影响。FPE经口给药（灌胃），每天一次，连续7天。将小鼠随机分为4组：对照组、模型组、FPE（100、200 mg/kg）组。酒精耐受量、酒精中毒时间、血酒精浓度、肝脏乙醇脱氢酶（ADH）、乙醛脱氢酶（ALDH）活性、血清天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）活性、肝、脑组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-px）、过氧化氢酶活性及丙二醛（MDA）生成量。结果：与模型组相比，FPE预处理组大鼠的酒精耐受时间明显延长，酒精中毒时间明显缩短，血酒精浓度降低，肝脏ADH、ALDH活性升高。FPE还能显著降低肝损伤指数和血清ALT、AST活性。此外，FPE预处理小鼠急性乙醇染毒后，肝脏MDA含量降低，GSH-px和过氧化氢酶活性升高，脑SOD和过氧化氢酶活性升高。此外，FPE预处理小鼠表现出显着缓解急性乙醇中毒后的记忆障碍。结论：本研究表明，FPE预处理可以增强酒精代谢，防止肝损伤，并减轻急性酒精中毒后的记忆障碍，这种效果可能与其调节酒精代谢和抗氧化酶。
Background: The effect of Flos Puerariae extract (FPE) on alcohol metabolism, hepatic injury, and memory impairment was assessed following acute ethanol (EtOH) intoxication in mice.Methods: The model of acute EtOH intoxication was established by intragastric administration with 8 g/kg EtOH in mice. FPE was orally administrated (gavage) once a day for 7 consecutive days. Mice were randomly divided into 4 groups: control group, model group, and FPE groups (100, 200 mg/kg). Alcohol tolerance and intoxication time, blood alcohol concentration, the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) in liver, aspartate amino transferase (AST) and alanine amino transferase (ALT) in serum, superoxide dismutase (SOD), glutathione peroxidase (GSH-px), catalase and the formation of malondialdehyde (MDA) in both liver and brain, as well as memory ability were determined after acute alcohol exposure.Results: Compared with model group, pretreatment with FPE significantly prolonged alcohol tolerance time and shortened intoxication time, which is accompanied by decreased blood alcohol concentration and elevated activities of ADH and ALDH in liver. Moreover, the index of hepatic injury, ALT, and AST activities in serum was markedly decreased by pretreatment with FPE. Additionally, decreased MDA level, enhanced GSH-px and catalase activities in liver, as well as enhanced SOD and catalase activities in brain were found in FPE pretreated mice after acute exposure to EtOH. Furthermore, FPE pretreated mice showed markedly relieved memory disruption following acute EtOH intoxication.Conclusions: This study suggests that FPE pretreatment could enhance alcohol metabolism, prevent hepatic injury, and relieve memory impairment after acute alcohol intoxication and that this effect is likely related to its modulation on the alcohol metabolizing and antioxidant enzymes.