Structure and sequence of human FGF8
Structure and sequence of human FGF8
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DOI：
10.1006/geno.1996.0349
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发表时间：
1996-07-01
期刊：
影响因子：
4.4
通讯作者：
MacArthur, CA
中科院分区：
文献类型：
--
作者：
Gemel, J;Gorry, M;MacArthur, CA
最近的证据表明，在重要胚胎结构的模式和产物中，在脊椎动物发育过程中表达了FGF8。对鼠基因的克隆和分析表明，至少有八个具有共同羧基区域的潜在蛋白质同工型，由外显子2和3编码，但具有不同的氨基末端，由多个5'外显子（外显子1a，1a，1a）编码的RNA替代剪接产生。 1B，1C和1D）。现在，我们报告人类FGF8基因的克隆和顺序。人FGF-8同工型与公共羧基区域中的鼠相同。人类的四种同工型与氨基末端中的鼠同工型相同或非常相似，但是，由于明显的序列差异，潜在的鼠同工型中的四个没有相应的人类同工型，从而导致外显子的读数阻滞剂。 1b的FGF8。人类缺乏四种鼠同工型提出了它们在鼠发育中的作用的问题。 （c）1996 Academic Press，Inc。
Recent evidence indicates that Fgf8 is expressed during vertebrate development in multiple locations involved in the patterning and outgrowth of important embryo structures. Cloning and analysis of the murine gene revealed at least eight potential protein isoforms that share a common carboxyl region, encoded by exons 2 and 3, but possess different amino termini, generated by alternative splicing of RNA encoded by multiple 5' exons (exons 1A, 1B, 1C, and 1D). We now report the cloning and sequence of the human FGF8 gene. Human FGF-8 isoforms are identical to their murine counterparts in the common carboxyl region. Four of the human isoforms are identical to, or very similar to, the murine isoforms in the amino termini, However, four of the potential murine isoforms do not have corresponding human isoforms due to marked sequence divergence, leading to a blocked reading frame in exon 1B of FGF8. The lack of the four murine isoforms in humans raises the question of their function in murine development. (C) 1996 Academic Press, Inc.