Relationship of Sequence and Phase Separation in Protein Low-Complexity Regions.
Relationship of Sequence and Phase Separation in Protein Low-Complexity Regions.
复制标题
蛋白质低复杂区域中序列和相位分离的关系。
DOI：
10.1021/acs.biochem.8b00008
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发表时间：
2018-05-01
期刊：
影响因子：
2.9
通讯作者：
Mittag T
中科院分区：
文献类型：
--
作者：
Martin EW;Mittag T
液-液相分离似乎通过形成生物分子凝聚物在细胞的区室化中发挥关键作用。在这些缩合物中发现了许多具有低复杂性区域的蛋白质，并且它们可以响应于温度、pH和离子浓度的变化而在体外进行相分离。因此，低复杂性区域可能是介导应激区室化的重要参与者。然而，相位行为如何编码在它们的氨基酸组成和模式中，人们知之甚少。我们在这里讨论，聚合物物理学提供了一个强大的框架，我们的理解的热力学的混合和分层，以及如何在初级序列的相行为编码。我们建议将低复杂度区域进一步分类为子类别，基于它们的序列属性和相位行为。正在进行的研究有望提高我们将低复杂性区域的主要序列与其相行为以及所产生的生物分子凝聚物的新兴可折叠性和材料特性联系起来的能力，从而为跨长度尺度的基本生物过程提供机械见解。
Liquid-liquid phase separation seems to play critical roles in the compartmentalization of cells through the formation of biomolecular condensates. Many proteins with low-complexity regions are found in these condensates, and they can undergo phase separation in vitro in response to changes in temperature, pH and ion concentration. Low-complexity regions are thus likely important players in mediating compartmentalization in response to stress. However, how the phase behavior is encoded in their amino acid composition and patterning is only poorly understood. We discuss here that polymer physics provides a powerful framework for our understanding of the thermodynamics of mixing and demixing and for how the phase behavior is encoded in the primary sequence. We propose to classify low-complexity regions further into sub-categories based on their sequence properties and phase behavior. Ongoing research promises to improve our ability to link the primary sequence of low-complexity regions to their phase behavior as well as the emerging miscibility and material properties of the resulting biomolecular condensates, providing mechanistic insight into this fundamental biological process across length scales.
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低复杂性蛋白段的原子结构揭示了组装网络的扭结β薄片。
DOI：
10.1126/science.aan6398
发表时间：
2018-02-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hughes MP;Sawaya MR;Boyer DR;Goldschmidt L;Rodriguez JA;Cascio D;Chong L;Gonen T;Eisenberg DS
通讯作者：
Eisenberg DS
影响因子：
15
作者：
Cho, Younhee;Sagle, Laura B.;Iimura, Satoshi;Zhang, Yanjie;Kherb, Jaibir;Chilkoti, Ashutosh;Scholtz, J. Martin;Cremer, Paul S.
通讯作者：
Cremer, Paul S.
影响因子：
56.9
作者：
Franzmann, Titus M.;Jahnel, Marcus;Alberti, Simon
通讯作者：
Alberti, Simon
影响因子：
21.8
作者：
Aumiller, William M., Jr.;Keating, Christine D.
通讯作者：
Keating, Christine D.
影响因子：
3.3
作者：
Asthagiri, D.;Karandur, Deepti;Pettitt, B. Montgomery
通讯作者：
Pettitt, B. Montgomery