Characterization of murine leukaemia virus-specific DNA present in normal mouse cells.
Characterization of murine leukaemia virus-specific DNA present in normal mouse cells.
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正常小鼠细胞中存在的小鼠白血病病毒特异性 DNA 的表征。
DOI：
10.1038/newbio244076a0
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发表时间：
1973
期刊：
影响因子：
--
通讯作者：
S. Aaronson
中科院分区：
文献类型：
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作者：
Lawrence D. Gelb;Julie B. Milstien;Malcolm A. Martin;S. Aaronson
从许多动物物种中分离到了RNAC型病毒。在小鼠和鸡中，它们以未表达的形式存在于每个细胞1-3中。从遗传学和生物化学的角度研究了内源性C型病毒的遗传信息是否整合到细胞基因组中。在小鼠细胞中检测到了生物可区分病毒的诱导性基因座，表明这些基因座可能代表病毒结构信息4，5。许多生化研究已经表征和量化了禽类和小鼠细胞中C型病毒特异性DNA 6-9。在一些报告中，通过测量未标记细胞DNA对标记DNA探针重新结合动力学的影响来量化每细胞中病毒DNA等价物的数量。使用体外制备的带有RNA肿瘤病毒逆转录酶的标记双链(DS)DNA，早期的报道表明，C型病毒DS DNA产物的大部分与相对较小部分的病毒基因组7，11互补；这种体外DNA探针不能有效地区分不同物种的DNA，因为存在病毒遗传信息7，8。部分DS DNA产物也被证明具有更能代表70年代基因组7，11的遗传复杂性。
RNA type C viruses have been isolated from many animal species. In the mouse and chicken, they exist in an unexpressed form in every cell1–3. Whether endogenous type C viral genetic information is integrated within the cell genome has been studied genetically and biochemically. Loci for inducibility of biologically distinguishable viruses have been detected in mouse cells, suggesting that the loci may represent virus structural information4,5. Numerous biochemical studies have characterized and quantified type C virus-specific DNA in avian and murine cells6–9. In some reports, the number of viral DNA equivalents per cell was quantified by measuring the effect of unlabelled cellular DNA on the reassociation kinetics of a labelled DNA probe10. Using labelled double stranded (ds) DNA prepared in vitro with the reverse transcriptases of RNA tumour viruses, earlier reports indicated that the major portion of the type C virus ds DNA product was complementary to a relatively small fraction of the viral genome7,11; this in vitro DNA probe did not effectively discriminate between the DNAs of different species with respect to the presence of viral genetic information7,8. A portion of the ds DNA product was also shown to have a genetic complexity more representative of the 70S genome7,11.